紫薯提取物抗氧化及延长寿命作用的研究  

EFFECT OF PURPLE SWEET POTATO EXTRACT ON ANTIOXIDANT FUNCTION AND LIFESPAN IN DROSOPHILA MELANOGASTER

作　　者：黄杰[1,2] 刘素稳[3] 马娜[1,2] 陈纯[1] 薛文琛 王浩[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457 [2]天津科技大学生物工程学院,天津300457 [3]河北科技师范学院食品科技学院,秦皇岛066004

出　　处：《营养学报》2015年第5期495-499,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学(青年)基金项目(No.31201322);"十二五"国家科技计划项目(No.2012BAD33B05);天津市高等学校科技发展基金计划项目(No.20100609);天津科技大学卓越人才实验班专项项目(No.1314A216)

摘　　要：目的探究紫薯提取物(purple sweet potato extract,PSPE)的抗氧化活性及延长寿命作用。方法以雄性果蝇为实验动物模型,采用生存试验与急性试验测定饲喂不同浓度(0,0.5,1.0,2.0 mg/ml)PSPE对果蝇寿命的影响,并测定果蝇体内抗氧化酶活力及抗氧化相关基因表达水平。结果 PSPE可以有效延长果蝇寿命,其中2.0mg/ml组果蝇的平均寿命较空白对照组延长了20.4%(P<0.05),且随着浓度的增大,呈剂量依赖关系。酶活测定结果显示,1.0、2.0mg/ml组果蝇体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力显著升高(P<0.05),丙二醛(MDA)含量显著下降(P<0.01)。Real time-PCR结果显示,1.0、2.0 mg/ml组果蝇体内SOD、CAT m RNA表达水平极显著上调(P<0.01),Methuselah(MTH)m RNA表达水平显著下调(P<0.05)。结论紫薯提取物可能通过上调抗氧化基因表达水平以提高抗氧化酶活力和抑制脂质过氧化反应,从而有效延长果蝇寿命。Objective To determine the effect of purple sweet potato extract （PSPE） on antioxidant function and lifespan in Drosophila melanogaster. Methods Male Drosophila were treated with different doses （0, 0.5, 1.0, 2.0mg/ml） of PSPE, then lifespan and challenge tests were carried out. Moreover, antioxidant enzymes activities and the mRNA expression levels of antioxidant-related genes were measured. Results Compared with the control, the lifespan of the Drosophila supplemented with PSPE was significantly prolonged （P〈0.05）. The mean lifespan was significantly extended by 20.4% （P〈0.05） in 2.0 mg/ml PSPE group and there was a dose-dependent relationship. In the Drosophila supplemented with 1.0, 2.0mg/ml PSPE, the activities of superoxide dismutase （SOD） and catalase （CAT） were increased significantly （P〈0.05）, while the content of malondialdehyde （MDA） decreased significantly （P〈0.01）. The mRNA expressions of SOD and CAT in the Drosophila supplemented with 1.0, 2.0mg/ml PSPE were up-regulated significantly （P〈0.01）, while the methuselah （MTH） mRNA level was significantly down-regulated （P〈0.05）. Conclusion PSPE can prolong the lifespan of Drosophila by up-regulating the expression of antioxidant genes, increasing the activities of antioxidant enzymes and inhibiting lipid peroxidation.

关 键 词：紫薯提取物 果蝇 抗氧化 寿命 

分 类 号：R285.5[医药卫生—中药学]