6 Gy 137Cs γ射线照射对小鼠造血功能损伤的动态观察研究  

Effects of 6 Gy 137Cs γ-irradiattion on the hematopoietic system of mice

作　　者：路璐[1] 张俊伶[1] 李德冠[1] 王月英[1] 孟爱民[2] 

机构地区：[1]天津市放射医学与分子核医学重点实验室,中国医学科学院放射医学研究所,300192 [2]中国医学科学院实验动物研究所,北京100021

出　　处：《国际放射医学核医学杂志》2015年第5期393-396,共4页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：国家重点基础研究发展计划（“973”项目）重大专项（2011CB964800-G）;国家自然科学基金面上项目（81372928）;协和青年基金资助和中央高校基本科研业务费专项资金资助项目（3332013044）;中央级公益性科研院所基本科研业务专项（1521）

摘　　要：目的 观察6 Gy 137Cs γ射线照射对小鼠造血功能的影响.方法 取雄性C57BL/6小鼠70只,随机分为对照组和照射组,每组35只.照射组小鼠接受6 Gy 137Cs γ射线一次性全身照射,分别于受照后1、3、7、10、14、35和56d测定小鼠体质量,并检测外周血象的变化、外周血网织红细胞百分率和骨髓有核细胞数,在受照后14、35和56 d检测小鼠骨髓细胞粒单系集落形成能力.结果 照射后小鼠体质量下降,14d后缓慢上升;外周血象中WBC和血小板数迅速下降,分别于照射后7和10d下降至最低值,而后缓慢回升;骨髓有核细胞数急剧下降,于照射后7和10d下降至最低值,而后逐渐恢复;外周血网织红细胞百分率经历了下降、恢复、超出正常值范围及恢复至正常值的过程;骨髓有核细胞粒单系集落形成能力显著下降.结论 6 Gy 137Cs γ射线照射可引起小鼠外周血象的变化并抑制造血祖细胞的增殖能力.Objective To observe the effects of 6 Gy 137Cs γ-irradiation on the hematopoietic system of mice.Methods Seventy male C57BL/6 mice were randomly divided into control and irradiated groups （n=35 each）.The irradiation group mice received 6 Gy mCs γ-irradiation.Bodyweight, peripheral blood cell counts, bone marrow mononuclear cell counts, and reticulocyte percentage were measured at 1, 3, 7, 10, 14, 35, and 56 d after total body irradiation.CFU-GM was detected at 14, 35, and 56 d after 6 Gy 137Cs γ-irradiation.Results After irradiation, the bodyweight of irradiated mice decreased but slowly increased after 14 d.The peripheral blood of irradiated mice had significant changes in WBC and platelet counts： it respectively decreased to the lowest value at 7 and 10 d post-irradiation and subsequently increased slowly.The reticulocyte percentage decreased, recovered, exceeded the normal range, and returned to normal.Furthermore, the bone marrow mononuclear cell counts decreased sharply, decreased to the minimum value after 7 d post-irradiation, and gradually recovered.The monophyletic cloning ability of irradiated mouse bone marrow nuclear granulocytes also decreased significantly.Conclusion 6 Gy 137Cs γ-irradiation could cause changes in the peripheral blood of mice and inhibit the proliferation of hematopoietic progenitor cells.

关 键 词：造血系统 全身照射 辐射损伤 实验性 小鼠 近交C57BL 

分 类 号：R818[医药卫生—放射医学]