盐炙对杜仲中京尼平苷酸体内药代动力学的影响  

Evaluation of Salt Processing on Pharmacokinetics of Geniposidic Acid in Eucommia ulmoides Oliver

作　　者：高倩倩[1,2] 翁泽斌[1,2] 赵根华 颜翠萍 陈志鹏[1,2] 蔡宝昌[1,2] 徐晗[4] 李伟东[1,2] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023 [2]南京中医药大学药学院,江苏南京210023 [3]济川药业集团股份有限公司临床医学部,江苏泰州225400 [4]安徽中医药大学药学院,安徽合肥230012

出　　处：《南京中医药大学学报》2015年第5期453-456,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省高校自然科学基金(11KJA360001);江苏省中药学优势学科开放课题(2011ZYX2-010);江苏省“青蓝工程”项目(2014)

摘　　要：目的建立测定大鼠血浆中京尼平苷酸的UHPLC-MS/MS方法,研究盐炙对杜仲中京尼平苷酸药代动力学的影响。方法色谱柱:BEH-C18(100mm×2.1mm,1.7μm);流动相:0.1%甲酸水-乙腈梯度洗脱;流速:0.3mL/min。结果京尼平苷酸在生品和盐炙品组大鼠体内的药动学参数分别为:AUC(0-t)(1 547.18±272.28)、(2 120.694±664.532)ng·h/mL;AUC(0-∞)(1 564.42±273.97)、(2 145.61±659.983)ng·h/mL;Cmax(517.59±51.24)、(733.292±261.34)ng/mL;MRT(2.68±0.11)、(2.551±0.08)h;T1/2(1.37±0.08)、(1.43±0.17)h;Tmax(1.52±0.1)、(1.51±0.1)h。结论杜仲盐炙后有助于促进京尼平苷酸的吸收,一定程度上增加了其生物利用度。ABSTRACTOBJECTIVE To establish UHPLC-MS/MS method to determine geniposidic acid in rat plasma and to investigate the effect of salt processing on pharmacokinetics of geniposidic acid in Eucommia ulmoides Oliver.METHODS Chromato-graphic columnBEH-C18100 mm＆×2.1 mm1.7 μmthe mobile phaseacetonitrile-0.1% formic acid aqueous solution. The gradient elution program was performed at a flow rate of 0.3 mL/min.RESULTS The main pharmacokinetic parameters of geniposidic acid in raw and salt products were thatAUC0-t were1 47.18±272.282 120.694± 664.532ng.h/mLAUC0-∞ were1 564.42±273.972 145.61 ±659.983ng.h/mLCmax were517.59±51.24733.292±261.34ng/mLMRT were2.68±0.112.551±0.08hT1/2 were1.37±0.081.43±0.17hTmax were1.52±0.11.51± 0.1h.CONCLUSION Salt processing can obviously enhance the absorption of geniposidic acid in Eucommia ulmoides Oliverand increase the bioavailability.

关 键 词：盐炙 杜仲 京尼平苷酸 药代动力学 

分 类 号：R283.1[医药卫生—中药学]