三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究  

San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A

作　　者：许岩磊[1] 陈曦琰 陈绪[1] 卞卫和[1] 姚昶[1] 朱小纾[2] 陈佳静[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]西悉尼大学生物和健康学院

出　　处：《南京中医药大学学报》2015年第5期469-474,共6页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81473676);江苏省高校优势学科建设项目Ⅱ期(012062003010);江苏省中医院高峰学术人才培养(y2014cr06)

摘　　要：目的探讨三黄煎剂对乳腺癌MCF-7、MDA-MB-231细胞凋亡的影响及Aurora激酶A(Aurora A)蛋白表达及功能的影响,并探讨其内在机制。方法采用CCK-8法检测三黄煎剂对乳腺癌细胞MCF-7、MDA-MB-231增殖的影响。AnnexinVFITC/PI法检测MCF-7、MDA-MB-231细胞凋亡率。q-PCR法检测MCF-7、MDA-MB-231细胞Aurora A、p53的mRNA表达水平。Western Blot法检测MCF-7、MDA-MB-231细胞凋亡相关蛋白的表达及Aurora A蛋白的表达。结果三黄煎剂对MCF-7、MDA-MB-231细胞增殖抑制率呈浓度梯度依赖增长(P<0.05),给药48h疗效好于24h(P<0.05),与给药72h无统计学差异(P>0.05)。三黄煎剂能够诱导MCF-7、MDA-MB-231细胞凋亡,并上调c-PARP、c-Caspase 3、Bax蛋白的表达,下调Bcl-2蛋白的表达,呈浓度梯度依赖。三黄煎剂能够下调Aurora A蛋白及mRNA的表达、上调p53蛋白及mRNA的表达。结论三黄煎剂能够通过下调Aurora A蛋白及mRNA的表达,抑制Aurora A的生物活性,抑制MCF-7、MDA-MB-231细胞增殖,诱导其凋亡。OBJECTIVE To explore the effect of San Huang decoction on the apoptosis of breast cancer cells and the effect on the mRNA and protein expression and function of Aurora kinase A and discuss the underlying mechanism of San Huang in-duced apoptosis.METHODS The inhibition of breast cancer cells proliferation was determined by CCK-8 assay.The apoptosis of breast cancer cells was detected by AnnexinV-FITC/PI Staining.The expression of mRNA of Aurora A was examined by q -PCR analysis.The expression of apoptosis-related proteins and Aurora A were determined by Western Blot analysis.RE-SULTS San Huang decoction inhibited the proliferation of breast cancer cells in a does-dependent mannerP 〈0.05.The effect of inhibition caused by San Huang decoction 48 hours after delivering to breast cancer cells was better than 24 hoursP 〈0.05 although similar as 72 hoursP 〉0.05.San Huang decoction was also found to induce apoptosis in both MCF-7 and MDA-MB-231 cell lines in a dose-dependent manner.Consistent with cellular resultsSan Huang decoction treatment signifi-cantly increased the apoptosis-related protein level of cleaved-PARPc-PARPcleaved-Caspase 3c-Caspase 3and Baxdown -regulated Bcl-2 in a does-dependent manner.MeanwhileSan Huang decoction decreased the mRNA and protein level of Auro-ra A and increased those of p53 in a does-dependent manner.CONCLUSION San Huang decoction at the first time was able to promote the apoptosis of breast cancer cells via inducing the suppression of Aurora A.

关 键 词：三黄煎剂 AURORA A 乳腺癌细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]