铁皮石斛对镉胁迫牛蛙红细胞诱变的缓解作用  

Alleviating Effects of Dendrobium officinale on Erythroecytes Variation of Bullfrog under Cadmium Stress

作　　者：张韵[1] 陈海霞[1] 张静[1] 李兴霞[1] 唐正义[1] 

机构地区：[1]内江师范学院生命科学学院,四川内江641100

出　　处：《内江师范学院学报》2015年第10期23-26,共4页Journal of Neijiang Normal University

基　　金：内江师范学院大学生科研项目(14NSD-30)

摘　　要：以牛蛙为材料,采用急性毒性实验方法,以蒸馏水配制成Cd2+浓度为0.0,0.5,1.0,2.0,4.0mg/L的染毒剂Ⅰ和铁皮石斛提取液配制成Cd2+浓度为0.0,0.5,1.0,2.0,4.0mg/L的染毒剂Ⅱ,研究了在染毒剂Ⅰ和染毒剂Ⅱ的胁迫下对牛蛙血红细胞的诱变效应.结果表明:染毒剂Ⅰ胁迫诱导牛蛙红细胞所得到的微核千分率,凋亡率,异形核率,呈先上升后下降的"抛物线"型,且分别在Cd2+浓度为2.0,2.0,1.0mg/L时有最大值,染毒剂Ⅱ胁迫牛蛙红细胞所产生的异形核率呈先上升后下降的"抛物线"型,且在Cd2+浓度为2.0mg/L时有最大值.凋亡率和微核千分率与Cd2+浓度呈剂量效应.染毒剂Ⅱ胁迫的红细胞的微核千分率,凋亡率,异形核率随Cd2+浓度的上升趋势较Cd2+单独胁迫牛蛙红细胞小.结论:铁皮石斛对重金属Cd2+胁迫的牛蛙红细胞有保护作用.An acute toxicity assay of cadmium were performed using bullfrog as the material. The concentrations of Cd^2＋ are 0. 0, 0. 5, 1.0, 2. 0, 4. 0 mg / L respectively either with distilled water （exposure agent I） or Dendrobium officinale extracts （exposure agent Ⅱ）. The mutagenic effect to the bullfrog erythrocytes under the stress of the exposure agent I or exposure agent Ⅱ were determined. The results showed that with the concentration of Cd^2＋ increase in exposure agent I, ratios of micronuclei, apoptosis and abnormal nuclei of the bullfrog erythrocytes firstly increase and then decrease as a parabolic curve. Peaks of these ratios were reached at concentration of Cd^2＋ 2. 0, 2.0 and 1.0 mg/L respectively. Exposure agent Ⅱ showed similar trend while ratios mentioned above reached maximal all at concentration of Cd^2＋ 2.0 mg/L. Dose effect between the Cd^2＋ concentration and the ratio of apoptotic cells or micronuclei was observed. In addition, exposure agent Ⅱ revealed less toxicity compared with exposure agent I in terms of micronuclei, apoptosis and abnormal nuclei ratios of the bullfrog erythrocytes. Therefore, Dendrobium officinale has protection effect over heavy metal stress such as Cd^2＋ on bullfrog erythrocytes.

关 键 词：铁皮石斛 镉 细胞凋亡 微核 异形核 牛蛙 

分 类 号：X592[环境科学与工程—环境工程]