两种方法联合检测梅毒螺旋体抗体的应用价值  

Application value of two methods combined detection of Treponema pallidum antibody

作　　者：刘静[1] 于静波[1] 王玉红[1] 赵汐渟[1] 齐志丹[1] 张明磊[1] 

机构地区：[1]沈阳军区总医院检验科,110016

出　　处：《检验医学与临床》2015年第20期3026-3027,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨化学发光(CLIA)法与梅毒螺旋体抗体明胶颗粒凝集试验(TPPA)对梅毒螺旋体抗体检测的应用价值。方法采用CLIA法对患者血清进行梅毒螺旋体抗体检测,收集阳性血清标本425例,阴性血清标本90例,用TPPA进行确认;根据测定S/CO值将标本分为4组,统计各组CLIA法与TPPA的符合率。结果根据CLIA法检测结果,将标本分为4组:A组(S/CO<1.20,n=90);B组(S/CO为1.20~10.00,n=139);C组(S/CO>10.00~20.88,n=124);D组(S/CO>20.88,n=162)。A^D组CLIA法与TPPA的检测符合率分别为100.0%、68.3%、92.7%、100.0%,B组的检测符合率最低,与其他3组比较差异有统计学意义(P<0.05)。结论CLIA法检测梅毒具有敏感性高,自动化程度高,适合大批量标本常规筛查,CLIA法检测阳性结果需进一步做TPPA进行确证。Objective To explore the application value of Treponema pallidum antibody detection by combining CLIA and TPPA.Methods Treponema pallidum antibodies in serum were detected by CLIA,and a total of 425 positive samples and 90 negative samples were collected;then the collected samples were confirmed by TPPA.According to the S/CO values,the samples were divided into four groups and the coincidence rate of CLIA and TPPA in each group was analyzed.Results According to the CLIA results,the samples were divided into 4groups：group A（S/CO1.20,n=90）,group B（S/CO〈 1.20-10.00,n=139）,group C（S/CO〉10.00-20.88,n=124）,group D（S/CO〉20.88,n=162）.The coincidence rates of CLIA and TPPA in group A,B,C,and D were 100.0%,68.3%,92.7%,and 100.0%,respectively.The coincidence rate in group B was the lowest,which was significantly different from the other three groups（P〈0.05）.Conclusion CLIA is a highly sensitive and automated method,which is suitable for clinical routine screening of a large number of specimens.The positive results of CLIA should be confirmed by TPPA.

关 键 词：梅毒螺旋体抗体 化学发光法 梅毒螺旋体抗体明胶颗粒凝集试验 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]