杭州市狭叶小羽藓遗传多样性研究  

On the Genetic Diversity of Haplocladium Angustifolium in Hangzhou City

作　　者：陆文怡[1] 张军 潘江杰 张晓勤[1] 吴玉环[1] 薛大伟[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]山东省蒙阴第一中学,山东蒙阴276200

出　　处：《杭州师范大学学报（自然科学版）》2015年第5期489-492,506,共5页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(30970188);杭州师范大学研究性实验教学项目(YJ201307);杭州师范大学实验室开放项目(SYSKF2014005)

摘　　要：以杭州市不同生境的8个狭叶小羽藓(Haplocladium angustifolium)居群共64个个体为实验材料,利用ISSR、SRAP和RAPD三种标记进行了遗传多样性分析,结果表明:用筛选出的43个引物对这64个个体进行扩增共得到127个位点,居群间的遗传分化为35.98%,遗传一致度为0.5305,表现出较高的遗传多样性.聚类分析表明,位于生态环境较好的西溪湿地与龙门古镇的两个居群与其他6个居群之间关系较远.The samples of different populations of the moss Haplocladium angustifolium were collected from eight habitats in Hangzhou.The genetic diversity of different populations was analyzed using ISSR,SRAP and RAPD markers.127 loci were derived from 43 markers.The total populations of the H.angustifolium,with the Nei gene diversity index（H）of 35.98%,Shannon index（I）as 0.5305,showed high genetic diversity among populations.According to the results of cluster analysis based on Nei＇s genetic distances,two populations distributed in Xixi Wetlands and Longmen Ancient Town,which located in good ecological environment,were far from the other six populations.

关 键 词：杭州 狭叶小羽藓 遗传多样性 分子标记 

分 类 号：Q94[生物学—植物学]