大理地区合并HIV双重感染者结核分枝杆菌MLVA法基因分型研究  

MLVA based Mycobacterium tuberculosis clinical isolates genotyping analysis from HIV co-infection patients in Dali area,China

作　　者：聂恒[1] 王旭东[1] 吴利先[1] 

机构地区：[1]大理学院基础医学院微生物学与免疫学教研室,大理671000

出　　处：《中国人兽共患病学报》2015年第10期923-926,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.81260456)~~

摘　　要：目的探讨MLVA基因分型法在云南大理地区合并HIV双重感染者结核分枝杆菌基因分型中的运用,初步研究其基因型特征。方法选取大理地区61株合并HIV双重感染者结核分枝杆菌临床分离株,采用聚合酶链反应(PCR)分别对VNTR位点进行检测分析,应用BioNumerics(6.6)软件进行聚类分析。结果共对61株合并HIV双重感染者的结核分枝杆菌的15个VNTR位点进行检测,呈现出明显的基因多态性。各个位点的分辨能力不同,其中MIRU26(0.839)最高,MIRU4(0.341)最低。经聚类分析,61株双重感染结核分枝杆菌菌株可分为5个基因群,61个基因型,以Ⅰ型占比例最大占51.6%(32/61);H37Rv减毒株在Ⅱ型。结论大理地区合并HIV双重感染者结核分枝杆菌VNTR基因存在明显多态性,主要流行菌群为北京家族基因型。A new method based on the multiple locus variable number tandem repeat analysis （MLVA） was applied for the genotyping of combined HIV Mycobacterium tuberculosis in Dali to investigate the genotyping and distribution pattern of Myco- bacterium tuberculosis clinical isolates with MLVA. Mycobacterium tuberculosis clinical isolates were selected from Dali area, and the polymorphism of VNTR locus was tested with PCR. The clustering of genotype was analyzed by BioNumerics（6.6）. Result showed that 15 VNTR loci of 61 combined HIV Mycobacterium tuberculosis clinical isolates were analyzed respectively. There were obvious polymorphisms of VNTRs. The discrimination power of these loci appeared different from each other, with the biggest Hunter-Gaston index （0. 839） loci was MIRU26, and the smallest one （0. 341） loci was MIRU4. The clustering of genotype showed that these strains could be categorized into 5 gene clusters and 61 genotype, the proportions of cluster I was the biggest one, 51.6% were cluster I which including 32 strains. The standard strain Ha7Rv was belongs to cluster 1I. Its indicated that there are obvious polymorphisms of VNTRs of combined HIV Mycobacterium tuberculosis clinical isolates in Da- li. The main genotype was Beijing family genotype.

关 键 词：结核分枝杆菌 基因分型 双重感染 HIV MLVA 

分 类 号：R378.91[医药卫生—病原生物学]