面神经损伤后面神经核中神经型钙黏附分子及胎盘型钙黏附分子的表达  

Expression of neuronal cadherin and placental cadherin in facial motoneurons after facial nerve injury

作　　者：李雷激[1] 徐超然[1] 覃纲[1] 刘跃华[1] 祝琳[1] 

机构地区：[1]泸州医学院附属医院耳鼻咽喉头颈外科,四川省泸州市646000

出　　处：《中国组织工程研究》2015年第37期5978-5982,共5页Chinese Journal of Tissue Engineering Research

基　　金：泸州市科技局立项课题(14135)~~

摘　　要：背景:面神经周围性损伤后,首先涉及其中枢神经元轴突的逆行性反应,神经能否再生则取决于神经元胞体的存活及功能状态。目的:检测面神经损伤后,面神经核中神经型钙黏附分子和胎盘型钙黏附分子的表达变化。方法:将新西兰大白兔随机分为模型组(n=48)和对照组(n=8)。模型组兔建立右侧面神经压榨损伤模型。模型组分别于损伤后1,4,7,14,21,28 d各取8只兔进行检测。运用免疫组织化学SP法及实时定量PCR法检测兔右侧面神经核运动神经元中神经型钙黏附分子和胎盘型钙黏附分子蛋白及mR NA的表达水平。结果与结论:对照组兔右侧面神经核运动神经元中无神经型钙黏附分子或胎盘型钙黏附分子标记的阳性神经元。模型组兔右侧面神经核运动神经元中存在神经型钙黏附分子和胎盘型钙黏附分子阳性神经元,2种阳性神经元数量均在第14天时达到峰值。与对照组相比,模型组损伤后4-28 d兔面神经核中神经型钙黏附分子mR NA的表达水平明显增加,损伤后1 d时兔面神经核中胎盘型钙黏附分子mR NA的表达水平明显下降,损伤后7-28 d时兔面神经核中胎盘型钙黏附分子mR NA的表达水平明显增加。提示面神经损伤的早期即出现2种分子的阳性表达,其中胎盘型钙黏附分子的表达自神经损伤后一直存在,而神经型钙黏附分子表达时间相对较短。在面神经损伤时,面神经核中神经型钙黏附分子和胎盘型钙黏附分子均表达增加,说明面神经再生可能与黏附分子的高表达有关。BACKGROUND;Peripheral facial nerve injury first involves the retrograde reactions of central nervous system axons,and nerve regeneration will depend on the survival and functional status of neuronal cell bodies.OBJECTIVE;To explore the expression of neuronal cadherin and placental cadherin in facial nuclei after facial nerve injury.METHODS;New Zealand white rabbits were randomly divided into model group（n=48） and control group（n=8）.In the model group,every eight rabbits were used to carry out the test respectively at 1,4,7,14,21 days after the model of facial nerve injury（right side） was established.SP and real-time quantitative PCR methods were taken to test the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus at m RNA and protein levels.RESULTS AND CONCLUSION;The control group had neuronal cadherin-and placental cadherin-positive neurons.In the model group,neuronal cadherin and placental cadherin positively expressed in the facial motorneurons（right side）,and their expressions were peaked at 14 days.Compared with the control group,the m RNA expression of neuronal cadherin in the facial motorneurons was increased significantly at 4-28 days afterinjury; the m RNA expression of placental cadherin in the facial motorneurons was decreased significantly at 1 day after injury,and then increased significantly at 7-28 days.It is suggested that the expression of neuronal cadherin and placental cadherin is positive in the early stage of facial nerve injury,and the expression of placental cadherin is always present,while the expression of neuronal cadherin relatively lasts for a short time.After facial nerve injury,the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus is both increased,which indicates that the facial nerve regeneration may be related to the high expression of adhesion molecules.

关 键 词：组织构建 组织工程 神经型钙黏附分子 胎盘型钙黏附分子 面神经核 信号转导 面神经再生 免疫组织化学 实时定量PCR 

分 类 号：R318[医药卫生—生物医学工程]