构建腰椎手术失败综合征大鼠模型及肾俞磁刺激治疗的可能机制  

Construction of rat models of failed back surgery syndrome and the possible mechanisms of Shenshu magnetic stimulation therapy

作　　者：吴峰[1,2] 陈宣煌[1,3] 黄漫为[1,2] 林海滨[1,3] 郑锦清[1,2] 

机构地区：[1]福建医科大学教学医院,南方医科大学附属莆田医院 [2]莆田学院附属医院康复医学科,福建省莆田市351100 [3]莆田学院附属医院骨科,福建省莆田市351100

出　　处：《中国组织工程研究》2015年第40期6413-6417,共5页Chinese Journal of Tissue Engineering Research

基　　金：福建省莆田学院科研基金项目(2014029);福建省莆田市科技计划课题(2013S03(5))~~

摘　　要：背景:腰椎手术失败综合征出现的重要原因是术后硬膜外瘢痕粘连,因此,探索预防腰椎术后硬膜外瘢痕粘连的方法一直是脊柱外科研究的热点。目的:观察肾俞穴磁刺激疗法对腰椎手术失败综合征模型大鼠硬膜外粘连的作用。方法:60只SD大鼠应用椎板切除法建立腰椎手术失败综合征模型,建模成功后均分为2组,肾俞磁疗组于造模后1周开始进行双侧肾俞穴磁刺激,1周治疗5 d,连续6周;空白对照组不干预。造模后13周取材,光镜下观察两组硬膜外瘢痕所占面积比,粘连范围大小,胶原纤维形成情况,并计数成纤维细胞数量;并比较两组瘢痕组织中羟脯氨酸含量及转化生长因子β1表达量。结果与结论:肾俞磁刺激治疗后,模型大鼠硬膜外瘢痕形成少,仅少数成纤维细胞浸润,瘢痕羟脯氨酸含量与转化生长因子β1表达量少,与空白对照组比较差异有非常显著性意义(P<0.001)。提示肾俞磁刺激疗法能明显减少腰椎术后硬膜外瘢痕形成,是其治疗腰椎手术失败综合征的可能机制。BACKGROUND： The important reason for failed back surgery syndrome is the postoperative epidural scar adhesions, therefore, exploring the methods of preventing postoperative lumbar epidural scar adhesions has always been a hot research in spine surgery field. OBJECTIVE： To investigate the effect of Shenshu point magnetic stimulation therapy on epidural scar adhesions in rat models of failed back surgery syndrome. METHODS： Sixty Sprague-Dawley rat models of failed back surgery syndrome were successfully established using the method of laminectomy, and then divided into Shenshu magnetic therapy group and blank control group Beginning from 1 week after modeling, rats in the Shenshu magnetic therapy group were subjected to bilateral Shenshu magnetic stimulation for 6 weeks, 5 days of treatment per week. Rats in the blank control group were not given any intervention. At 13 weeks after modeling, rats were harvested and the area ratio of epidural scars, range of adhesions and formation of collagen fibers were observed under light microscope. Fibroblasts were counted. Hydroxyproline content and transforming growth factor β1 expression in scar tissue were compared between these two groups.RESULTS AND CONCLUSION： After Shenshu magnetic stimulation therapy, epidural scar tissue was reduced,a few fibroblasts infiltrated and hydroxyproline content and transforming growth factor β1 expression in the epidural scar tissue were decreased. There were very significant differences about these indices between Shenshu magnetic therapy and blank control groups（P 〈0.001）. These results suggest that Shenshu magnetic stimulation therapy can significantly reduce postoperative lumbar epidural scar formation, which is the possible mechanism in the treatment of failed back surgery syndrome.

关 键 词：实验动物 骨及关节损伤动物模型 硬膜外瘢痕 粘连 肾俞 磁刺激 羟脯氨酸 转化生长因子β1 成纤维细胞 

分 类 号：R318[医药卫生—生物医学工程]