高压氧干预脑梗死模型大鼠脑水肿及神经功能变化  

Variations of brain edema and neurological function of rat models of cerebral infarction after hyperbaric oxygen therapy

作　　者：田烜 

机构地区：[1]沧州市人民医院神经内二科,河北省沧州市061001

出　　处：《中国组织工程研究》2015年第40期6423-6428,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究认为,高压氧有较好保护脑神经和脑细胞的作用,应用高压氧可使氧分压快速弥撒到相对缺氧的脑组织中,增加脑组织的血氧含量,促进脑水肿及脑神经功能的恢复。目的:观察大脑中动脉阻塞造模后高压氧干预对大鼠脑梗死组织水肿的影响,并探讨其对脑梗死大鼠神经功能保护的可能作用机制。方法:成年雌性SD大鼠65只,造模成功60只,随机区组法分为假手术组、脑梗死组、高压氧组,每组20只,按照线栓线法建立大鼠大脑中动脉阻塞脑梗死模型。造模后3 d,通过TUNEL法检测各实验组大鼠脑梗死区神经细胞的凋亡情况。伤后72 h通过RT-PCR、Western blot检测脑梗死区周围AQP4/9、基质金属蛋白酶9/2基因转录和蛋白的表达,通过苏木精-伊红染色观察脑梗死区病理组织形态学变化,通过免疫组织化学法检测胶质纤维酸性蛋白的表达量,高压氧干预后24 h,3 d及伤后1、2周行Longa行为学评分,检测神经功能的损伤情况。结果与结论:1高压氧组Longa行为学评分在治疗后1,2 d均较脑梗死组显著降低(P<0.05)。2造模后3 d高压氧组细胞凋亡指数均明显低于脑梗死组(P<0.05)。3造模后72 h,与脑梗死组相比高压氧组AQP4/9、基质金属蛋白酶9/2基因和蛋白表达均较显著降低(P<0.05)。结果提示高压氧治疗通过减少大鼠脑梗死区神经细胞的凋亡和降低脑组织水肿,对脑梗死起到保护作用。BACKGROUND： Several studies have suggested that hyperbaric oxygen could better protect cranial nerve and brain cells. Hyperbaric oxygen therapy can make oxygen partial pressure rapidly diffusing toward relatively hypoxic brain tissue, so as to increase blood oxygen content in the brain tissue, reduce brain edema and promote the recovery of brain function. OBJECTIVE： To observe the effects of hyperbaric oxygen therapy on brain tissue swelling in rats after middle cerebral artery occlusion, and discuss the possible mechanism of action underlying the neuroprotective effects of hyperbaric oxygen therapy in rats with cerebral infraction. METHODS： Sixty adult female rat models of cerebral infarction were successfully established by middle cerebral artery occlusion using suture method and then randomly divided into the sham, cerebral infarction and hyperbaric oxygen therapy groups（n=20 rats/group）. At 3 days after middle cerebral artery occlusion, apoptosis of nerve cells in the infract area of rats in each group was detected by TUNEL method. At 72 hours after middle cerebral artery occlusion, the gene transcription and protein expression of aquaporin 4/9 and matrix metalloproteinases 9/2 in the peri-infarct area were detected by RT-PCR and western blot analysis. The pathomorphological change in the infract area was observed by hematoxylin-eosin staining. The expression level of glial fibrillary acidic protein was detected by immunohistochemistry. At 24 hours and 3 days after hyperbaric oxygen therapy and at 1 and 2 weeks after middle cerebral artery occlusion, neurological behaviors were evaluated using Longa behavioral scores. RESULTS AND CONCLUSION： After 1, 2 days of hyperbaric oxygen therapy, Longa behavioral scores in the hyperbaric oxygen therapy group were significantly lower than those in the cerebral infarction group（P〈0.05）. At 3 days after middle cerebral artery occlusion, cell apoptosis index in the hyperbaric oxygen therapy group was significantly lower than that in the cerebral infarctio

关 键 词：实验动物 脑及脊髓损伤动物模型 神经功能 大鼠 

分 类 号：R318[医药卫生—生物医学工程]