高脂喂养2型糖尿病下肢缺血模型大鼠的构建  

Construction of type 2 diabetes rat models of lower limb ischemia by feeding with high-fat diet

作　　者：李晓玲[1] 刘颐轩[2] 朱旅云[1] 宋光耀[3] 王振全[4] 王超[2] 

机构地区：[1]解放军白求恩国际和平医院内分泌科,河北省石家庄市050082 [2]河北省人民医院风湿免疫科,河北省石家庄市050000 [3]河北省人民医院内分泌科,河北省石家庄市050000 [4]解放军白求恩国际和平医院普通外科,河北省石家庄市050082

出　　处：《中国组织工程研究》2015年第40期6429-6435,共7页Chinese Journal of Tissue Engineering Research

基　　金：河北省自然科学基金(H2013505067)~~

摘　　要：背景:目前普遍采用结扎或切除大鼠后肢股动脉或髂动脉的方法来制备后肢缺血模型,但对于如何建立及评估糖尿病高脂高血糖慢性动脉硬化闭塞症的缺血状态,尚无一个稳定有效慢性缺血模型及方法。目的:构建及评价高脂喂养2型糖尿病下肢缺血模型大鼠。方法:将大鼠20只随机分为2组,糖尿病组10只予以高脂喂养6个月,腹腔注射链硫脲菌素50 mg/kg诱发糖尿病模型,对照组10只予以普食喂养6个月,两组大鼠均结扎股动脉建立右下肢缺血模型。结果与结论:建模后第1天,两组大鼠多普勒血流及CT血管造影均呈显著降低提示缺血造模成功,两组建模后第7,14天行激光多普勒检测发现血流有逐渐恢复趋势,建模后第28天糖尿病组血流恢复较对照组显著迟缓(P<0.05)。CT血管造影检测显示,建模后第28天,糖尿病组右下肢股动脉结扎处近端仅有少量血管代偿性增加,远心端仍无明显血流。病理组织及免疫组织化学染色染色显示,建模后第28天,糖尿病组缺血部位肌肉组织出现结构组织破坏,炎性细胞浸润,毛细血管密度患侧低于健侧;糖尿病组缺血肌肉组织血管内皮生长因子较对照组的蛋白表达明显增加(P<0.05)。结果显示,长期高脂喂养糖尿病大鼠,通过股动脉结扎及CT血管造影技术可成功构建糖尿病下肢缺血模型。BACKGROUND： The method of ligating or resecting rat lower limb femoral or iliac artery has been widely used to make rat models of lower limb ischemia, but there have been no stable and effective methods used to establish diabetic models of chronic atherosclerotic occlusive diseases and to evaluate the ischemic status of hindlimbs of models. OBJECTIVE： To establish type 2 diabetes rat models of lower limb ischemia by feeding with high-fat diet and to evaluate them. METHODS： Twenty rats were randomly and evenly divided into diabetes group（n=10） and control group（n=10）. Rats in the diabetes group were fed with high-fat diet for 6 months, and were intraperitoneally injected with streptozotocin（50 mg/kg） to induce diabetes. Rats in the control group were fed with normal diet for 6 months. Rats in these two groups were subjected to ligation of femoral artery to establish right lower limb ischemia models. RESULTS AND CONCLUSION： At the first day of modeling, color Doppler flow imaging and CT angiography showed obviously decreased blood flow suggesting the success of establishing ischemia model. At 7 and14 days after modeling, the blood flow of rats in these two groups showed a gradual recovery as detected by color Doppler flow imaging. At 28 days after modeling, blood flow of rats in the diabetes group was significantly slower than that in the control group（P〈0.05）. CT angiography showed that at 28 days after modeling, only a small amount of compensatory increase in blood flow of the blood vessels at the ligation position of proximal right lower limb femoral artery was seen in the diabetes group, while no obvious blow was observed in the distal part. Histopathologic and immunohistochemical staining showed that at 28 days after modeling, destroyed tissue structure and inflammatory cell infiltration were observed in the ischemic region and capillary density on the affected side was lower than that on the healthy side. The protein expression of vascular endothelial growth factor in the ischemic mu

关 键 词：组织工程 糖尿病 模型 动物 股动脉 实验动物 心肺及血管损伤动物模型 糖尿病血管病变 高脂饮食 SD大鼠 下肢缺血 激光多普勒灌注成像 CT血管成像 血管新生 股动脉结扎 河北省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]