建立复发性口疮大鼠模型和黄芩苷的干预  

Construction of rat models of recurrent aphthous ulcer and the intervention of baicalin

作　　者：秦蕴[1] 

机构地区：[1]黑龙江大庆油田总医院口腔正畸科,黑龙江省大庆市163001

出　　处：《中国组织工程研究》2015年第40期6504-6508,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:细胞外基质的降解与重构是多种病理及生理过程的共同通路,参与了多种口腔疾病的发病过程。目的:分析复发性口疮大鼠细胞凋亡的细胞外基质重构参与作用及黄芩苷的干预作用,为相关新药开发和临床治疗提供参考。方法:40只SD大鼠随机分为4组:对照组、复发性口疮模型组、小剂量黄芩苷组和大剂量黄芩苷组。后3组大鼠采用口腔黏膜匀浆组织加弗氏免疫佐剂建立大鼠复发性口疮模型。小剂量黄芩苷组和大剂量黄芩苷组大鼠分别给予10 mg/kg和100 mg/kg黄芩苷灌胃,1次/d,连续14 d。结果与结论:与对照组相比,模型组大鼠血清超氧化物歧化酶水平明显降低,而丙二醛水平明显增高,口腔黏膜中Bax、Caspase3和基质金属蛋白酶2/9表达水平增加,而Bcl-2表达水平降低。而小剂量黄芩苷组和大剂量黄芩苷组大鼠上述指标明显恢复,且不同剂量黄芩苷的影响差异有显著性意义。提示黄芩苷主要通过抑制细胞外基质降解与重构过程治疗复发性口疮。BACKGROUND： Extracellular matrix degradation and remodeling is the common pathway of a variety of pathological and physiological processes, and is invovled in the pathogenesis of a variety of oral diseases. OBJECTIVE： To analyze the role of extracellular matrix remodeling following cellular apoptosis in recurrent aphthous ulcer and the therapeutic effect of baicalin, so as to provide references for new drug development and clinical treatment. METHODS： Forty Sprague-Dawley rats were randomly divided into four groups： control, recurrent aphthous ulcer model, low-dose baicalin and high-dose baicalin groups. Rat models were established using oral mucosal homogenate plus Freund＇s immunoadjuvants in the recurrent aphthous ulcer model, low-dose baicalin, and high-dose baicalin groups. Rats in low-dose baicalin and high-dose baicalin groups were intragastrically administered 10 mg/kg and 100 mg/kg of baicalin respectively for 14 days, once per day. RESULTS AND CONCLUSION： Compared with the control group, serum superoxide dismutase level was distinctly decreased, however, malondialdehyde level distinctly increased; the expression levels of Bax, Caspase3 and matrix metalloproteinase 2/9 were increased, but the expression of Bcl2 was decreased in the recurrent aphthous ulcer model group. The abovementioned indicators of rats returned greatly towards normal levels in the low dose baicalin and high dose baicalin groups, and there was significant difference in the effects of different doses of baicalin on these indicators. These results suggest that baicalin treats recurrent aphthous ulcer mainly through inhibiting extracellular matrix degradation and remodeling.

关 键 词：实验动物 口腔损伤及修复动物模型 复发性口疮 细胞外基质降解 重构 基质金属蛋白酶 黄芩苷 弗氏免疫佐剂 

分 类 号：R318[医药卫生—生物医学工程]