苯丙酸诺龙对烫伤模型大鼠雄激素受体介导靶基因转录调控的影响  

Effect of nandrolone phenylpropionate on androgen receptor-mediated transcriptional regulation of target genes in rat scald models

作　　者：李凯 岑瑛[2] 

机构地区：[1]核工业416医院整形美容科,四川省成都市610051 [2]四川大学华西医院烧伤整形科,四川省成都市610041

出　　处：《中国组织工程研究》2015年第40期6509-6514,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:中重度烧创伤一直是临床全身治疗的难点,与创面局部处理并重,极大影响治疗预后。同化激素在烧伤动物模型与临床患者的使用治疗中取得安全良好效果。同化激素的应用虽受运动兴奋剂管理的限制,但其雄激素受体与核受体辅助调节因子仍是近年来基因调控机制研究的热门新兴领域。目的:探索苯丙酸诺龙对烫伤大鼠体内雄激素受体介导靶基因转录调控的影响。方法:将36只大鼠随机分为苯丙酸诺龙组、模型组与对照组。苯丙酸诺龙组和模型组大鼠以热水制成20%体表面积深Ⅱ度烫伤大鼠模型后2 d,分别后肢肌肉注射苯丙酸诺龙和生理盐水,隔日1次,连续21 d。结果与结论:苯丙酸诺龙组与模型组大鼠肝脏及性腺(睾丸、卵巢)中类固醇受体辅助活化因子1和胰岛素样生长因子1基因表达水平差异有显著性意义(P<0.05,除肝脏中类固醇受体辅助活化因子1);而模型组与对照组大鼠肝脏及性腺(睾丸、卵巢)中类固醇受体辅助活化因子1、c-myc和胰岛素样生长因子1基因表达水平差异无显著性意义(P>0.05)。说明在不同组织不同生理病理条件下苯丙酸诺龙对类固醇受体辅助活化因子1、c-myc、胰岛素样生长因子1基因表达的作用是不同的。BACKGROUND： Moderate to severe burn and trauma, treatment of which has been paid equal attention to wound surface, is always a difficulty of clinical systemic treatment and has a poor prognosis. Anabolic hormones have acquired secure and good results in the treatment of burns in both animals and clinical patients. Although use of anabolic hormones is restricted by the doping management, but its androgen receptor and nuclear receptor coregulators are the newly emerging areas of interests in the field of gene regulation mechanism in recent years. OBJECTIVE： To investigate the effect of nandrolone phenylpropionate on androgen receptor-mediated transcriptional regulation of target gene in rat scald models. METHODS： Thirty-six rats were randomly divided into nandrolone phenylpropionate, model and control groups. In the nandrolone phenylpropionate and model groups, rats were subjected to a 20% total body surface area second-degree scald injury by hot water. Two days after model preparation, rats in these two groups were intramuscularly injected with nandrolone phenylpropionate and saline, once every other day, for a total of 21 days. RESULTS AND CONCLUSION： The gene expression levels of steroid receptor coactivator-1 and insulin-like growth factor 1 in the rat livers and gonads（testes, ovaries） were significantly different between the nandrolone phenylpropionate and model groups（P〈0.05）, except steroid receptor coactivator-1 in the liver. There were no significant differences in steroid receptor coactivator-1, c-myc and insulin-like growth factor 1 in the rat livers and gonads（testes, ovaries） between model and control groups（P〈0.05）. The results demonstrate that nandrolone phenylpropionate has different effects on the gene expression of steroid receptor coactivator-1, c-myc and insulin-like growth factor 1 in different tissues under different physiological and pathological conditions.

关 键 词：实验动物 组织构建实验模型 烫伤 类固醇受体辅助活化因子1 C-MYC 胰岛素样生长因子1 

分 类 号：R318[医药卫生—生物医学工程]