改进SD-Wistar大鼠肾移植慢性排斥模型的造模方法  

An experimental model of chronic renal allograft rejection in SD-Wistar rats

作　　者：余鹏程[1] 刘永光[2] 郭颖[2] 李民[2] 肖宗宇[3] 胡孔和[4] 黄进军[5] 辛军[6] 吴志强[2] 赵明[2] 

机构地区：[1]福建医科大学附属泉州第一医院肾内科,福建省泉州市362000 [2]南方医科大学珠江医院器官移植中心,广东省广州市510282 [3]南方医科大学珠江医院神经外科,广东省广州市510282 [4]南方医科大学珠江医院骨科,广东省广州市510282 [5]南方医科大学珠江医院整形外科,广东省广州市510282 [6]福建医科大学附属泉州第一医院泌尿外科,福建省泉州市362000

出　　处：《中国组织工程研究》2015年第40期6520-6525,共6页Chinese Journal of Tissue Engineering Research

基　　金：泉州市科技计划项目(2009Z39)~~

摘　　要：背景:国际上常用的肾移植慢性排斥模型是Fisher→Lewis大鼠肾移植模型等,但这些模型在国内均较难以获得并且价格昂贵,大规模使用受到了一定的限制。目的:探索建立SD→Wistar大鼠肾移植慢性排斥模型的新方法。方法:将56对SD→Wistar大鼠作左肾原位移植,受体自身右肾保留作为内对照。23只成功移植的受体大鼠随机分为模型组(n=15)和对照组(n=8)。模型组大鼠在移植后给予10 d的小剂量环孢素微乳化剂[2 mg/(kg·d),腹腔注射],对照组大鼠未给予免疫抑制治疗。结果与结论:对照组大鼠所有移植肾均在4周内出现不可逆的急性排斥反应,移植肾坏死;模型组大鼠移植后4,8,12周时均可见移植肾中出现中等度的炎症细胞浸润,在移植后12周内出现典型的慢性排斥组织病理学改变,其Banff总分随移植后时间的延长而升高。2组大鼠所有受体的自身右肾均没有出现组织病理学改变。模型组大鼠移植后第4天环孢素浓度谷值为(153.2±17.1)μg/L。说明通过给予肾移植的SD→Wistar大鼠受体10 d小剂量的环孢素微乳化制剂(2 mg/kg),可使移植肾出现中等度的炎症细胞浸润并于12周内形成慢性排斥的典型组织病理学改变,可作为研究肾移植慢性排斥的动物模型。BACKGROUND： Fisher-Lewis rat kidney transplant models are the international common chronic renal allograft rejection models, but their application is greatly limited because of difficulty in model preparation and high costs. OBJECTIVE： To explore a new method of establishing SD-Wistar rat models of chronic renal allograft rejection. METHODS： Fifty-six pairs of SD-Wistar rats were subjected to left kidney orthotopic transplantation. The right kidneys of the recipients were intact and used as internal controls. 23 rat recipients were randomly divided into model group（n=15） and control group（n=8）. The rats in the model group were injected with cyclosporine microemulsion for 10 days（2 mg/kg/day, i.p.） after kidney transplantation. The rats in the control group were not treated with immunosuppressive therapy.RESULTS AND CONCLUSION： The irreversible acute rejection occurred in all the transplanted kidneys of rats in the control group within 4 weeks, leading to the necrosis of transplanted kidney. Moderate inflammatory cell infiltration appeared in the transplanted kidneys of rats in the model group at 4, 8 and 12 weeks after transplantation. Typical histopathological changes of chronic rejection were observed within 12 weeks after transplantation. The Banff total scores were increased with time after transplantation. All these histopathological changes were not observed in the intact right kidneys of rat recipients in both groups. The valley value of cyclosporine concentration in the transplanted rats in the model group was（153.2±17.1） μg/L at 4 days after transplantation. These results demonstrate that after a short course treatment of cyclosporine microemulsion（2 mg/kg/day for 10 days, i.p.） in SD-Wistar rat recipients, moderate inflammatory cell infiltration was followed by development of chronic rejection within 12 weeks, and such animal models can be used for studying chronic renal allograft rejection.

关 键 词：实验动物 组织构建实验模型 大鼠 慢性排斥 组织病理学 环孢素 

分 类 号：R318[医药卫生—生物医学工程]