建立脊髓横断模型大鼠的系统评价  

The systematic evaluation of establishing spinal cord transection model in rats

作　　者：金昕[1] 周宾宾[2] 李波霖[1] 杨程程[2] 张鸿升[1] 

机构地区：[1]广西中医药大学,广西壮族自治区南宁市530001 [2]广西中医药大学第一附属医院,广西壮族自治区南宁市530001

出　　处：《中国组织工程研究》2015年第40期6553-6560,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81460746)~~

摘　　要：背景:建立一种成功率较高、安全可靠的标准脊髓横断模型是研究脊髓修复的前提条件。目的:评价大鼠脊髓横断模型制备的价值及椎板切除对脊髓的影响。方法:检索美国国立医学图书馆(PubM ed)、中国知网(CNKI)、重庆维普(VIP)、万方数据库中所用关于大鼠脊髓横断模型的随机对照研究。结果与结论:11篇随机对照研究符合纳入标准(英文2篇,中文9篇),共394只大鼠纳入研究,脊髓半横断组与椎板切除组1-6周内下肢运动功能评分(BBB评分)、4周内电生理差异有显著性意义(WMD=-12.86,95%CI-16.10至-9.62,P<0.01)、(WMD=15.36,95%CI 11.36-19.36,P<0.01),半横断组6周后BBB评分差异无显著性意义(WMD=-10.28;95%CI-24.20-3.64;P=0.15);脊髓全横断组与椎板切除组1-6周内BBB评分、4周内电生理差异有显著性意义(WMD=-18.83,95%CI-20.64至-17.01,P<0.01)、(WMD=-11.21,95%CI-16.35至-6.08,P<0.01)。椎板切除组与正常组4周内BBB评分与电生理评分差异无显著性意义(WMD=-0.00,95%CI-0.01-0.01,P=1)、(WMD=0.43,95%CI-0.35-1.21,P=0.28);大鼠脊髓横断组和椎板切除组死亡数差异无显著性意义(RD=0.05,95%CI-0.03-0.13;P=0.26)。说明脊髓横断法是一种稳定性好、可复制性强、存活率高的脊髓损伤研究模型,但其横断的准确性、术后护理及对照组的设立有待商榷。BACKGROUND： Establishing a highly successful, safe, reliable standard spinal cord transection model is the precondition of studying spinal cord injury repair. OBJECTIVE： To evaluate the value of preparing spinal cord transection model in rats and the effects of laminectomy on spinal cord. METHODS： We searched the randomized controlled trials involving rat models of spinal cord transection in the databases of PubMed, CNKI, VIP and WanFang. RESULTS AND CONCLUSION： 11 randomized controlled trials （RCTs） met the inclusion criteria （two in English, 9 in Chinese）, and a total of 394 rats were included in the study. There were significant differences in the lower limb motor function scores （BBB scores） within 1-6 weeks after injury （WMD=12.86, 95%CI 16.10 to -9.62, P 〈 0.01 ） and electrophysiological indices within 4 weeks after injury （WMD=15.36, 95%CI11.36 to 19.36, P 〈 0.01） between spinal cord hemisection group and laminectomy group. The BBB scores after 6 weeks were not significantly different between these two groups （WMD=-10.28; 95%CI-24.20 to 3.64; P=-0.15）. There were significant differences in the lower limb motor function scores （BBB scores） within 1-6 weeks after injury （WMD= 18.83, 95% CI 20.64 to -17.01, P 〈 0.01） and electrophysiological indices within 4 weeks after injury （WMD= 11.21, 95%CI-16.35 to -6.08, P 〈 0.01 ） between spinal cord transection group and laminectomy group. No significant differences were found in BBB scores （WMD= 0.00, 95%CI 0.01 to 0.01, P=1） and electrophysiotogical indices （WMD=0.43, 95%CI=0.35 to 1.21,P=0.28） within 4 weeks after injury between laminectomy group and normal group. There was no significant difference in the number of deaths between spinal cord hemisection group and iaminectomy group （RD=0.05, 95%CI=0.03 to 0.13; P=0.26）. Experimental findings indicate that spinal cord transection is a method of inducing spinal cord injury with good stability, strong replication and high survival rate. But transection a

关 键 词：实验动物 脑及脊髓损伤动物模型 Meta分析 大鼠 电生理 椎板切除 死亡数 BBB评分 

分 类 号：R18[医药卫生—流行病学]