葛根素抑制人成骨样MG-63细胞分泌IL-6的分子机制研究  

Molecular mechanism for inhibitory effect of puerarin on IL-6 secretion in human osteoblast-like MG-63 cells

作　　者：罗婷[1] 李乐飞[1] 郭小芹[2] 张立朋[2] 王越[2] 李玲[3] 韩泽民[4] 

机构地区：[1]辽宁医学院武警后勤学院附属医院研究生培养基地,辽宁锦州121000 [2]武警后勤学院病原生物和免疫学教研室,天津300309 [3]武警后勤学院药理学教研室,天津300309 [4]武警后勤学院附属医院口腔教研室,天津300162

出　　处：《中国药理学通报》2015年第11期1524-1529,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81273520;81572852;81502256);天津市自然科学基金资助项目(No12JCZDJC26300;15JCYBJC28900);武警后勤学院科学技术研究项目(No WHZ201202;WHB201404;WHB201405;WHB201406;2015ZXKF05)

摘　　要：目的研究葛根素(puerarin)抑制人成骨样MG-63细胞分泌IL-6的分子机制。方法利用兼有两种雌激素受体(estrogen receptor,ER)亚型表达的人成骨样MG-63细胞为研究模型,通过ELISA、RT-PCR、荧光素酶报告基因检测、小RNA干扰等方法,研究葛根素对MG63细胞产生骨吸收调节因子IL-6的作用及作用机制。结果葛根素可明显抑制MG-63细胞IL-6表达和IL-6基因启动子的转录活性,并且该作用可被纯ER阻断剂ICI 182,780部分阻断。采用小RNA干扰技术进一步证明葛根素对成骨细胞的上述作用是ERα依赖的。结论葛根素调节人成骨细胞产生IL-6主要是ERα依赖的。Aim To investigate the molecular mecha-nism for the inhibitory effect of puerarin on IL-6 secre-tion in human osteoblast-likeMG-63 cells. Methods According to our previous studies, human osteoblast-like MG-63 cells containing two estrogen receptor ( ER) isoforms are a suitable model for this study. En-zyme-linked immnosorbent assay (ELISA), RT-PCR, luciferase reporter assay and small interfering RNA were performed to investigate the effect of puerarin on IL-6 expression in osteoblast-derived cells and underly-ing molecular mechanism. Results Puerarin could obviously inhibit IL-6 expression and IL-6 promoter ac-tivity by human osteoblastic MG-63 cells. Treatment with the ER antagonist ICI 182,780 abrogates the a-bove actions of puerarin on osteoblast-derived cells. U-sing siRNA technology, we further demonstrated that the effects of puerarin on IL-6 production were media-ted by ERα. Conclusion The effect of puerarin on IL-6 production in osteoblast is mediated by ERα.

关 键 词：葛根素 人成骨细胞 白细胞介素-6 雌激素受体Α 雌激素受体Β 小RNA干扰技术 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]