氟代苯硼酸与单糖间结合规律探究  

Investigation of Interactions between Fluorophenylboronic Acids and Monosaccharides

作　　者：吕辰晨 刘震[1] 

机构地区：[1]南京大学化学化工学院,生命分析化学国家重点实验室及生命化学协同创新中心,南京210093 [2]北京微量化学研究所,北京100091

出　　处：《分析化学》2015年第10期1459-1464,共6页Chinese Journal of Analytical Chemistry

基　　金：国家杰出青年科学基金(No.21425520);国家自然科学基金面上项目(No.21275073)资助~~

摘　　要：取代硼酸与顺式二羟基化合物间的相互作用是设计亲和配基、功能材料和传感元件以分离、富集和识别顺式二羟基化合物的重要基础。氟代苯硼酸在较低p H条件下具有较高的硼亲和力,但其与单糖间的相互作用规律目前尚未得到阐明。本研究以亲和毛细管电泳为相互作用表征手段,研究了9种不同结构的氟代苯硼酸与3种典型单糖(甘露糖、果糖和葡萄糖)在不同p H条件下的结合常数。同时,利用紫外分光光度法测量了这9种氟代苯硼酸的酸度系数。经过结构-性能关联,对氟代苯硼酸与甘露糖、果糖和葡萄糖的结合规律以及对葡萄糖的选择性进行了探讨。The interactions between boronic acids and cis-diol containing compounds are important basis for the design of affinity ligands,functional materials and sensors for the separation,enrichment and recognition of cisdiol containing biomolecules. Fluorophenylboronic acids（ FPBA） are a class of boronic acids with high affinity at relatively low p H. However,their interactions with monosaccharides have not been elucidated well yet. By using affinity capillary electrophoresis,herein we studied the association constants between nine different FPBA and three typical monosaccharides（ glucose,mannose and fructose） at different p H values. We also measured the p Kavalues of the FPBA using the UV-vis spectrophotometry. Through data comparison,the dependence of the binding strength on the structure of FPBA as well as the selectivity of glucose against fructose was examined.

关 键 词：氟代苯硼酸 单糖 相互作用 亲和毛细管电泳 

分 类 号：O627.31[理学—有机化学]