不同培养方法对相似穿孔线虫致病力的影响  

Influence of different culture methods on pathogenicity of Radopholus similis

作　　者：武玉环[1,2] 徐春玲[1] 张超[1] 黄欣[1] 彭晓放 谢辉[1] 

机构地区：[1]华南农业大学植物线虫研究室/华南农业大学植物检疫线虫检测与防疫研究中心,广州510642 [2]河北北方学院农林科技学院,张家口075000

出　　处：《华中农业大学学报》2015年第6期49-54,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31071665);广东省植物防疫检疫科研项目(2012ky002)

摘　　要：利用从进口观赏植物上截获的相似穿孔线虫6个种群,采用胡萝卜愈伤组织长期培养和温室盆栽的方法,观察不同培养方法对相似穿孔线虫致病力的影响。结果表明:在胡萝卜愈伤组织上长期培养和在寄主植物上接种复壮后的相似穿孔线虫均对红掌有致病性,但致病力有差异并发生变化;接种线虫后红掌根部发病率最低的为24.40%、最高的为90.00%,繁殖倍数最低的为0.20、最高为2.80;在胡萝卜愈伤组织上长期培养后6个种群的致病力降低,在寄主植物上接种复壮后致病力会增强,但至少要连续复壮2代致病力才能稳定。The influence of different culture methods on the pathogenicity of six populations of Radopholus similis from imported ornamental plants were tested on carrot disks and with pot experiment in greenhouse.R.similis can spread virulence to Anthurium andraeanum with both long-term culture on carrot disks and rejuvenation on A.andraeanum,but the influence of the two methods on the pathogenicity of R.similes were different.The lowest incidence of root was 24.4% while the highest was90.0%,the lowest productive factors was 0.20 while the highest was 2.80.The pathogenicity of six R.similis populations were reduced after long-term culturing on carrot disks,and can be stronger after rejuvenation on the host plant,but the pathogenicity would only be steady after continuous 2rejuvenations.

关 键 词：相似穿孔线虫 胡萝卜愈伤组织 致病力 红掌 

分 类 号：S432.45[农业科学—植物病理学]