通窍开玄法对鼻窦炎大鼠鼻黏膜水通道蛋白相关基因影响研究  

Research on the Influence of TongQiao KaiXuan Method on AQP-related Gene of Nasal Mucosa in Rats with Nasosinusitis

作　　者：敬樱[1] 张天娥[1] 罗再琼[1] 赵静[1] 张勤修[2] 杨九一[1] 

机构地区：[1]成都中医药大学基础医学院,四川成都611137 [2]成都中医药大学附属医院

出　　处：《西部中医药》2015年第8期8-11,共4页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81072727);四川省教育厅重点资助项目(编号:09ZA022)

摘　　要：目的:应用基因芯片技术分析通窍开玄法对鼻窦炎模型大鼠鼻黏膜水通道蛋白(AQP)的影响,并探讨其治疗鼻窦炎的生物学机制。方法:实验大鼠随机分为实验组、模型组和正常组,每组10只,实验组和模型组据Y.Gel的改良方法造模,正常组不做任何处理。造模成功后实验组分别给予三和通窍开玄汤低剂量组、三和通窍开玄汤中剂量组、三和通窍开玄汤高剂量组、青霉素V钾片灌胃7天,处死动物取鼻黏膜组织,应用Agilent基因芯片检测各组大鼠鼻黏膜水通道蛋白基因表达,并进行显著性分析。结果:中药各组均上调AQP1表达,中剂量组、高剂量组又上调AQP2,青霉素V钾组上调AQP12b基因;4组均下调AQP9基因,中药低剂量组还可下调AQP2、AQP12b,青霉素V钾组还可下调AQP1、AQP2。结论:三和通窍开玄汤与青霉素V钾片均对鼻窦炎具良好治疗效果,但调控的AQP相关基因表达机制不同,且中药组作用更为广泛,表明对AQP相关基因的调控可能是通窍开玄法治疗鼻窦炎生物学机制之一,AQP是玄府重要实质之一。Objective： To analyze the effects of Tong Qiao Kai Xuan method on aquaporin（AQP） of nasal mucosa in rat model with nasosinusitis by gene chip technology, and discuss its biological mechanism of treating nasosinusitis. Methods： The rats were randomized into the experiment group, the model group and the normal group, the rats in the experiment group and the model group were prepared by Y.Gel reforming method, the normal group were unhandled. After successfully establishing the models, the experiment group accepted intragastric administration of SanHe Tong Qiao Kai Xuan Tang in high, moderate and low dose, and phenoxymethylpenicillin potassium tablets for seven consecutive days, nasal mucosa was obtained from the animals, the AQP expressions of the rats in all the groups were detected with Agilent gene chip, and analyzed significantly. Results： AQP1 expressions raised in the groups of TCM,AQP2 expressions raised in moderate and high doses groups, AQP12 b gene was up-regulated in phenoxymethylpenicillin potassium tablets group; AQP9 gene was down-regulated in four groups, AQP2 and AQP12 b decreased in low dose groups of TCM, AQP1 and AQP2 were down-regulated in phenoxymethylpenicillin potassium tablets group.Conclusion： San He Tong Qiao Kai Xuan Tang and phenoxymethylpenicillin potassium tablets show better clinical effects in treating nasosinusitis, but the mechanisms of regulating AQP-related gene expression are different, and the functions of TCM groups are extensive, which demonstrates the regulation of AQP-related genes might be one of biological mechanisms of Tong Qiao Kai Xuan method in treating nasosinusitis, AQP is one of the important substances of the sweat pores.

关 键 词：鼻窦炎 基因芯片 通窍开玄法 水通道蛋白 

分 类 号：R285-5[医药卫生—中药学]