Comparative Studies on Sperm Ultrastructure of Three Gecko Species, Gekko japonicus, Gekko chinensis and Hemidactylus bowrigii(Reptilia, Squamata, Gekkonidae)  

作　　者：Shuangli HAO Liangliang PAN Zhouxi FANG Yongpu ZHANG 

机构地区：[1]College of Life and Environmental Science,Wenzhou University [2]College of Life Science,Wenzhou Medical University

出　　处：《Asian Herpetological Research》2015年第3期189-198,共10页亚洲两栖爬行动物研究（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China (31170376)

摘　　要：We provide the first description of the ultrastructure of the spermatozoa of Gekko japonicus, Gekko chinensis and Hemidactylus bowrigii for further understanding of the phylogenetic relationships of Gekkonidae. Mature spermatozoa of the three species differ in the occurrence and shape of epinuclear electron-lucent zone, nuclear space, neck cylinder and mitochondria. G. japonicus and G. chinensis have similar spermatozoan ultrastructure while H. bowrigii differs from these two species. In addition, these three species have neck cylinder with mitochondria in neck region and dense bodies arranged in grid with mitochondria in the midpiece, which may be the autapomorphies of the family Gekkonidae. Statistical analyses reveal that： total length of the spermatozoa was significantly different between G. japonicus and G. chinensis, as well as between G. japonicus and H. bowrigii（F2,57 = 23.66, P 〈 0.0001）; G. japonicus and H. bowrigii differ in head length（F2,43 = 4.64, P 〈 0.05） and the width of nuclear base（F2,22 = 3.97, P 〈0.05）. In addition, the midpiece length（F2,33 = 23.66, P 〈 0.01） of the spermatozoa was significantly different between H. bowrigii and G. japonicus, and also between H. bowrigii and G. chinensis. Lengths of perforatorium, acrosomal complex and nuclear rostrum and the width of nuclear shoulder are similar in all three species. Our results indicated that the sperm ultrastructure contained intra and intergeneric variabilities which is helpful for better understanding their genetic relationships.We provide the first description of the ultrastructure of the spermatozoa of Gekko japonicus, Gekko chinensis and Hemidactylus bowrigii for further understanding of the phylogenetic relationships of Gekkonidae. Mature spermatozoa of the three species differ in the occurrence and shape of epinuclear electron-lucent zone, nuclear space, neck cylinder and mitochondria. G. japonicus and G. chinensis have similar spermatozoan ultrastructure while H. bowrigii differs from these two species. In addition, these three species have neck cylinder with mitochondria in neck region and dense bodies arranged in grid with mitochondria in the midpiece, which may be the autapomorphies of the family Gekkonidae. Statistical analyses reveal that： total length of the spermatozoa was significantly different between G. japonicus and G. chinensis, as well as between G. japonicus and H. bowrigii（F2,57 = 23.66, P 〈 0.0001）; G. japonicus and H. bowrigii differ in head length（F2,43 = 4.64, P 〈 0.05） and the width of nuclear base（F2,22 = 3.97, P 〈0.05）. In addition, the midpiece length（F2,33 = 23.66, P 〈 0.01） of the spermatozoa was significantly different between H. bowrigii and G. japonicus, and also between H. bowrigii and G. chinensis. Lengths of perforatorium, acrosomal complex and nuclear rostrum and the width of nuclear shoulder are similar in all three species. Our results indicated that the sperm ultrastructure contained intra and intergeneric variabilities which is helpful for better understanding their genetic relationships.

关 键 词：SPERMATOZOA ultrastructure GEKKONIDAE phylogenetic relationship 

分 类 号：Q954.43[生物学—动物学]