基于再生纤维素皮肤修复材料的制备及其性能  

Regenerated cellulose materials for skin repair: preparation and performance

作　　者：邓立欢[1] 

机构地区：[1]惠州市第三人民医院烧伤外科,广东省惠州市516002

出　　处：《中国组织工程研究》2015年第38期6098-6103,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:再生纤维素膜具有很好的吸湿性和机械强度且生物降解性优良,但其韧性较差,有研究结果表明再生纤维素膜干膜的伸长率不足15%。目的:制备复合型2,3-二醛纤维素/胶原蛋白膜,并检测其机械性能、溶胀性能、透湿性和保水率等性能。方法:采用高碘酸钠氧化改性制备2,3-二醛纤维素/胶原蛋白膜,采用红外光谱和扫描电子显微镜观察其形态结构,并检测其机械性能、溶胀性能、透湿性和保水率,同时以再生纤维素膜、2,3-二醛纤维素膜为对照;将对数生长期小鼠胚胎成纤维细胞株接种于2,3-二醛纤维素/胶原蛋白膜上培养,1,3,5 d后采用MTT法检测细胞增殖。结果与结论:红外光谱证实胶原蛋白成功固化到2,3-二醛纤维素膜表面;扫描电镜显示,2,3-二醛纤维素/胶原蛋白膜具有疏松的多孔结构,胶原蛋白的固定使材料表面部分具有多孔网络结构,孔隙率为93.4%。2,3-二醛纤维素/胶原蛋白膜的机械性能、溶胀率、透湿性均强于2,3-二醛纤维素膜、再生纤维素膜(P<0.05),3组之间保水率差别不大。小鼠胚胎成纤维细胞在再生纤维素/胶原蛋白膜表面黏附紧密,形态伸展并呈现出不断增殖的趋势。表明2,3-二醛纤维素/胶原蛋白膜具有良好的机械性能、溶胀性能、透湿性及保水率,以及细胞相容性。BACKGROUND： The regenerated cellulose membrane has good moisture absorption and mechanical strength as well as good biodegradability, but its toughness is poorer. Studies have shown that the dry film of regenerated cellulose membrane has a poor elongation rate that is less than 15%. OBJECTIVE： To prepare the compound regenerated cellulose membrane/collagen membrane, and to test its mechanical properties, swelling properties, moisture permeability and water retention. METHODS： Using sodium periodate oxidation method, the 2,3-dialdehydecellulose/collagen membrane was prepared. Infrared spectroscopy and scanning electron microscope were used to observe the morphological structure, and test its mechanical properties, swelling properties, moisture permeability and water retention. Meanwhile, the regenerated cellulose membrane and 2,3-dialdehydecellulose membrane served as controls. Fibroblasts from logarithmic phase mouse embryos were seeded onto the 2,3-dialdehydecellulose/collagen membrane, and after 1, 3, 5 days, MTT method was used to detect cell proliferation. RESULTS AND CONCLUSION： Infrared spectra showed that the collagen was cured to the surface of 2,3-dialdehydecellulose membrane. Under the scanning electron microscopy, the 2,3-dialdehydecellulose/ collagen membrane had loose porous structure, with a porosity of 93.4%. The mechanical properties, swelling rate and moisture permeability of the 2,3-dialdehydecellulose/collagen membrane were significantly stronger than those of the 2,3-dialdehydecellulose membrane and regenerated cellulose membrane（P〈0.05）. However, there was no difference in the water retention rate among the three groups. Mouse embryonic fibroblasts tightly adhered to the surface of regenerated cellulose/collagen membrane, and presented a proliferative trend. Thesefindings indicate that the 2,3-dialdehydecellulose/collagen membrane has good mechanical properties, swelling property, moisture permeability, water retention, and cytocompatibility.

关 键 词：生物相容性材料 纤维素 生物敷料 生物材料 材料相容性 再生纤维素 胶原蛋白 伤口敷料 细胞相容性 

分 类 号：R318[医药卫生—生物医学工程]