两种脱敏剂封闭牙本质小管的扫描电镜观察  

Sealing effects of two desensitizers on the dentinal tubule: a scanning electron microscope observation

作　　者：李娜[1] 朱立江 王香兰 郑照杰 

机构地区：[1]潍坊医学院口腔医学院,山东省潍坊市261000 [2]青岛市口腔医院保健科,山东省青岛市266071

出　　处：《中国组织工程研究》2015年第38期6150-6154,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:MS Coat、极固宁脱敏剂均可以有效封闭牙本质小管,隔绝外界刺激,降低牙本质敏感。国内外学者对两者效果的对比多为描述性、定性研究。目的:通过扫描电镜定量观察MS Coat、极固宁脱敏剂对牙本质小管的封闭效果,并做出比较。方法:将30颗人离体新鲜第三磨牙样本制成3 mm厚的牙本质盘,以17%EDTA液体处理牙本质表面2 min,随机均分为3组,空白对照组不做任何处理,MS Coat组和极固宁组分别用MS Coat脱敏剂和极固宁脱敏剂对牙本质表面进行处理,采用扫描电镜观察各组牙本质表面和纵剖面结构。结果与结论:1表面观察:空白对照组牙本质小管全部开放;MS Coat组绝大部分牙本质小管口被堵塞;极固宁组牙本质小管口覆盖不规则鳞状晶体物质,仍可见一小部分暴露的牙本质小管口,但直径变窄。2纵剖面观察:空白对照组牙本质小管呈条状平行排列,无任何堵塞物;MS Coat组小管口有沉淀物沉积,较致密,基本上封闭了牙本质小管口;极固宁组牙本质小管内有鳞片状晶体物质沉积。组间牙本质直径及小管数量比较:空白对照组>极固宁组>MS Coat组(P<0.05)。表明MS Coat脱敏剂和极固宁脱敏剂均能有效封闭牙本质小管,且MS Coat脱敏剂的封闭效果优于极固宁。BACKGROUND： Both Ms Coat and Green Or TM desensitizers can seal dentinal tubules effectively, isolate the dentinal tubule from external stimuli and also reduce the dentinal hypersensitivity. The scholars around the world have discussed their effects from the descriptive and qualitative view. OBJECTIVE： To quantitatively assess and compare the sealing effects of Ms Coat and Green Or TM desensitizers on dentine tubules. METHODS： Thirty fresh human third molar samples were made into 3-mm dentin specimens that were treated with 17% EDTA for 2 minutes and randomly divided into three groups： Ms Coat group, Green Or TM group and control group with no treatment. In the former two groups, MS Coat desensitizer and Green Or TM desensitizer were applied respectively on the dentin surfaces. Scanning electron microscope was used to observe the dentin surface and longitudinal sections. RESULTS AND CONCLUSION：（1） Surface observation： In the control group, all of the dentinal tubules were exposed; in the MS Coat group, it was observed that almost all the dentinal tubules were obliterated; in the Green Or TM group, the dentin tubules were covered with irregular crystalline substance and only a small number of exposed dentine tubules were visible but narrowed obviously.（2） Longitudinal profile observation： In the control group, the dentin tubules were strip-shaped and arranged in parallel without any blockage; in the MS coat group, the tubules were covered with dense sediments, and the dentinal tubules were basically closed; in the Green Or TM group, there were scaly crystalline material deposited within the dentinal tubules. Dentinal diameter andnumber of dentinal tubules were ranked as follows： control group〉 Green Or TM 〉group MS Coat group（P〈0.05）. Both Ms Coat and Green Or TM desensitizers can occlude the tubules effectively, and moreover, the MS Coat is superior to the Green Or TM.

关 键 词：牙本质脱敏剂 牙本质 坑缝封闭物 生物材料 口腔生物材料 生物活材料 脱敏剂 牙本质小管 封闭 扫描电镜 

分 类 号：R318[医药卫生—生物医学工程]