注射型纳米壳聚糖骨形态发生蛋白2复合体诱导牙周组织的再生  

Injectable nano/chitosan/bone morphogenetic protein-2 induces periodontal tissue regeneration

作　　者：巴格那[1,2] 陈华荣[1,2] 李婷[1,2] 谢富强[1] 鱼灵会[3] 

机构地区：[1]兰州大学第二医院,甘肃省兰州市730000 [2]兰州大学口腔医学院,甘肃省兰州市730000 [3]兰州大学第一医院东岗院区口腔科,甘肃省兰州市730000

出　　处：《中国组织工程研究》2015年第38期6184-6189,共6页Chinese Journal of Tissue Engineering Research

基　　金：兰州市科技计划项目(2011-2-50);甘肃省科技支撑计划项目(1011FKCA103)~~

摘　　要：背景:壳聚糖水凝胶具有良好的生物兼容性、生物可降解性及抗菌性等特点,具有促进组织愈合和成骨诱导的作用,作为支架材料载负生长因子可保证外源生长因子的缓慢高效释放。目的:观察可注射性纳米壳聚糖骨形态发生蛋白2复合体促进大鼠牙周组织再生的效果。方法:将54只Wistar大鼠随机均分为3组,建立第二磨牙慢性牙周炎模型,建模成功后,实验组于第二磨牙牙周内植入可注射性纳米壳聚糖骨形态发生蛋白2复合体,对照组于第二磨牙牙周内植入可注射性纳米壳聚糖凝胶,空白组牙周内不植入任何药物。术后3,6,9周,进行牙龈出血指数、探诊深度、松动度、X射线片及组织病理学切片观察。结果与结论:实验组术后9周的探诊深度、松动度均低于对照组及空白组(P<0.05)。术后9周,实验组牙槽骨高度修复再生至根分叉处,骨小梁致密,分布均匀,可见大量牙骨质样结构、牙周韧带生成,新生牙槽骨致密且均匀分布在骨缺损区域,修复效果明显优于对照组及空白组。表明可注射性纳米壳聚糖骨形态发生蛋白2复合体具有抗炎和引导牙周组织再生的作用。BACKGROUND： Chitosan hydrogel has good biocompatibility, biodegradability and antibacterial property, which can promote tissue healing and induce bone formation. As a scaffold carrying growth factors, it can ensure the efficient and slow release of exogenous growth factors. OBJECTIVE： To observe the effect of injectable nano/chitosan/bone morphogenetic protein-2 composite to promote periodontal tissue regeneration in rats. METHODS： Fifty-four Wistar rats were randomized into three groups, and then chronic periodontitis model of the second molar was established. After modeling, injectable nano/chitosan/bone morphogenetic protein-2 composite was implanted into the periodontal tissue of the second molar in the experimental group; injectable nano/chitosan hydrogel was implanted in the control grouop; and nothing was implanted in the blank group. At 3, 6, 9 weeks after surgery, gingival bleeding index, probing depth, and tooth mobility were detected. X-ray and histopathological observations were carried out. RESULTS AND CONCLUSION： At 9 weeks after surgery, the probing depth and tooth mobility were both lowerin the experimental group than the other two groups（P〈0.05）. In the experimental group, the alveolar bone height was restored at the root bifurcations, bone trabeculae were arranged densely and evenly, the newly formed periodontal ligament and alveolar bone tissues were dense and equally distributed in the bone defect area at 9 weeks after surgery, indicating a better restoration effect than the control and blank groups. These findings suggest that the injectable nano/chitosan/bone morphogenetic protein-2 composite has an anti-inflammatory role and can guide periodontal tissue regeneration.

关 键 词：壳聚糖 凝胶类 骨形态发生蛋白类 生物材料 纳米材料 可注射性 纳米壳聚糖凝胶 骨形态发生蛋白2 牙周组织再生 

分 类 号：R318[医药卫生—生物医学工程]