血管张应力体外加载装置的研制  

The development of an in vitro loading device for vascular tensile stress

作　　者：雷亚楠[1] 丁皓[1,2] 兰海莲 杨乾[1] 刘志琳[3] 

机构地区：[1]上海理工大学医疗器械与食品学院,上海市200093 [2]上海健康医学院,上海市200093 [3]上海市第一人民医院,上海市200080

出　　处：《中国组织工程研究》2015年第38期6205-6210,共6页Chinese Journal of Tissue Engineering Research

基　　金：上海市教育委员会科研创新项目(13YZ148)~~

摘　　要：背景:国内外已经研制出多种体外细胞张应力加载装置,主要拉伸方法有矩形基底拉伸法、圆形基底变形法和4点弯曲梁加载法3种,其中圆形基底变形法虽能够很好的反映体内如肺泡的扩张、血管的脉动等真实情况,但该种加载过程中膜的应变是辐射对称的;4点弯曲梁加载法能够提供的应变范围很小,加载时间有限,应变调节比较困难。目的:采用矩形基底拉伸法研制血管张应力体外加载装置。方法:采用机电一体化设计研制血管张应力体外加载装置,由电源模块、控制模块、传动模块和数据采集模块4个部分组成,以硅胶片为基底材料,通过对电机旋转角度和转动速度的高精度控制,实现对硅胶膜片上的拉伸控制。结果与结论:通过测试和试验,该装置可以满足试验所需的参数范围,能够在体外模拟出人体张应力环境,初步认为该张应力加载装置的研制是成功的,实现了:1装置有两种工作模式:应力模式和应变模式,解决了基底加载装置的硅胶片材还没有实现标准化的问题。2能实现张应力在0-5×105 Pa范围内的调节。3能实现张应变在0-40%范围内的调节。4能实现0-80次/min的拉伸频率的变化,并能控制拉伸时间。BACKGROUND： After years of development, various in vitro loading devices for vascular tension stress have been created both at home and abroad, mainly including rectangular base stretching method, circular base deformation method and four-point bending beam load method. Although the circular base deformation method can well reflect the real situations in vivo such as the expansion of the alveoli and vascular pulsation, the strain on the membrane is actinomorphic. The four-point bending beam load method can just bring limited strain range and load time, along with a difficult strain regulation. OBJECTIVE： To develop an in vitro loading device for vascular tension stress using the rectangular base stretching method. METHODS： This in vitro loading device for vascular tension stress developed according to mechatronics design consisted of power supply module, control module, drive module and data acquisition module. The device couldachieve the tensile control on silicon diaphragm by high-precision control of the motor rotation angle and rotational speed. RESULTS AND CONCLUSION： Through tests and experiments, the device could meet the required range of parameters and simulate in vitro human tensile stress environment, which is preliminarily considered to develop successfully, achieving that：（1） two work patterns： stress mode and strain mode so as to solve the standardization of silicone substrate as loading device;（2） tensile stress can be adjusted in a range of 0-5×105 Pa;（3） tensile strain can be adjusted in 0-40% range;（4） stretching frequency can be in the regulation of 0-80 times/min and the stretching time can be controlled.

关 键 词：心血管疾病 硅胶 生物材料 材料相容性 张应力 张应变 加载装置 弹性基底 

分 类 号：R318[医药卫生—生物医学工程]