美洲大蠊脱脂膏对小鼠免疫功能的影响  

Effect of Periplaneta americana de-fat Extract on Immune Function in Mice

作　　者：李娇 张冰清 刘光明 夏盟凯 张小东 刘晓波 

机构地区：[1]大理大学药学与化学学院,云南大理671000 [2]云南省昆虫医药研发重点实验室,云南大理671000

出　　处：《大理学院学报（综合版）》2015年第10期1-5,共5页Journal of Dali University

基　　金：云南省重点产业创新工程项目(2008IF012)

摘　　要：目的:研究美洲大蠊脱脂膏(PAE)对正常小鼠和环磷酰胺造成的免疫低下小鼠免疫功能的影响。方法:对C57BL/6小鼠腹腔注射环磷酰胺制备免疫低下模型,PAE高、中、低剂量(4 g/kg,2 g/kg,1 g/kg)灌胃15 d,测定各组的T、B淋巴细胞的增殖能力,脏器质量和脏器指数,外周血细胞数目,骨髓有核细胞计数等。结果:PAE可以促进脾淋巴细胞的增殖功能,提高免疫低下小鼠的脏器质量和脏器指数,改善其外周血象,以及增加骨髓有核细胞的数目从而提高其免疫功能。结论:PAE可以改善免疫低下小鼠的免疫功能,而对正常小鼠的免疫功能几乎没有作用。Objective： To research the effect of Periplaneta americana de-fat extract on immune function in normal mice and in immunocompromised mice caused by cyclophosphamide. Methods： In this study, immunosuppressive model was established in C57BL/6mice by cyclophosphamide. After the high, medium and low dose（4 g/kg, 2 g/kg, 1 g/kg）of P. americana de-fat extract were orally administered for 15 d, T and B lymphocyte proliferation was measured. And the organ weights and organ index, the number and classification of peripheral blood cell, bone marrow nucleated cell were tested. Results：P. americana extract can promote the peoliferrative function of splenic linphocytes, increase the weight of immunocompromised mice organs and organ index, improve their peripheral blood, and increase the number of bone marrow cells to enhance their immune function. Conclusion： The P. americana defat extract can improve and influence the immune function on immunocompromised mice, but it has no effect on the immune function of normal mice.

关 键 词：美洲大蠊脱脂膏 免疫调节 T、B淋巴细胞增殖 脏器指数 外周血细胞 

分 类 号：R285.5[医药卫生—中药学]