寿胎丸调节树突状细胞功能治疗URSA作用与机制研究  

Study of effect and molecule mechanism of Shou Tai Decoction on URSA treatment by regulating function of dendritic cells

作　　者：赖楠楠 李紫薇[1] 王丽[1,2] 郭钰琪[1,2] 姚成芳[1,2] 李霞[1,2] 

机构地区：[1]山东省医学科学院基础医学研究所,济南大学山东省医学科学院医学与生命科学学院,济南250062 [2]山东省医学科学院基础医学研究所,山东省医药卫生肿瘤免疫与中药免疫重点实验室,山东省罕少见病重点实验室,卫生部生物技术药物重点实验室,国家中医药管理局免疫药理(三级)实验室,山东省免疫生物靶向药物工程技术中心,济南250062

出　　处：《中国免疫学杂志》2015年第10期1337-1341,1346,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(No.81373670);山东省自主创新成果转化专项(2013ZHZX2A0405);山东省科技发展计划项目(No.2012GSF11840);山东省计生委项目(No.[2014]14);山东省中管局项目(2013-216,2013-218);济南市科技明星计划项目(济科合字2012)

摘　　要：目的:探讨补肾固冲中药复方寿胎丸(STD)调节复发性自然流产患者(URSA)树突状细胞(DC)功能与机制。方法:正常早孕妇女和URSA患者各30例,作为正常对照组和URSA组,后者给予寿胎丸中药治疗。空腹抽取正常对照组及URSA患者治疗前后静脉血,分离单个核细胞,流式细胞术检测CD11c+HLA-DR+、CD11c+CD80+、CD11c+CD86+细胞比例,RT-PCR法检测静脉血HLA-DR、CD80、CD86、吲哚胺2,3-双加氧酶(IDO)m RNA转录水平,Western blot法检测IDO蛋白表达,ELISA法检测血清IL-12p70、IL-6蛋白水平。结果:与正常妊娠妇女比较,URSA患者外周血CD11c+HLA-DR+、CD11c+CD80+、CD11c+CD86+细胞比例表达显著升高(P<0.05),HLA-DR、CD80、CD86 m RNA转录水平显著升高(P<0.05),IDO m RNA及蛋白表达明显降低(P<0.05),血清IL-12p70、IL-6蛋白水平显著升高(P<0.01)。与寿胎丸治疗前比较,治疗后URSA患者外周血CD11c+HLA-DR+、CD11c+CD80+、CD11c+CD86+细胞比例及HLA-DR、CD80、CD86 m RNA转录水平表达显著降低(P<0.05),IDO m RNA及蛋白表达明显升高(P<0.05),血清IL-12p70、IL-6蛋白水平显著降低(P<0.01)。结论:URSA患者存在DC数量与功能改变,补肾安胎中药复方寿胎丸能调节DC功能,诱导母胎免疫耐受,治疗URSA。Objective：To investigate the role of dendritic cells（DC） in unexplained recurrent spontaneous abortion（URSA） and to study the effect and molecular mechanism of Traditional Chinese Medicine Shou Tai Decoction （ STD ） on URSA treatment by regulating the function of DC.Methods：30 cases of normal pregnancy women and 30 cases of URSA patients were taken as control group and URSA group respectively.URSA patients were treated with Shou Tai Decoction.Peripheral blood mononuclear cells were taken from both control group and URSA group before and after STD administration.The proportion of CD11c＋HLA-DR＋cells,CD11c＋CD80＋cells and CD11c＋CD86＋cells in peripheral blood were measured by flow cytometry.Moreover,the mRNA expression of HLA-DR,CD80,CD86 and Indoleamine2,3-dioxygenase（ IDO） in venous blood were detected by RT-PCR assay.The protein expression of IDO was detected by Western blot.Furthermore, the cytokines, including IL-12p70 and IL-6, in the blood serum were measured by ELISA.Results：Compared with normal pregnancy women,the proportion of CD11c＋HLA-DR＋,CD11c＋CD80＋,CD11c＋CD86＋cells and the mRNA expression of HLA-DR,CD80,CD86 of URSA patients in peripheral blood were both increased significantly（P〈0.05）, while the mRNA and protein expression of IDO were decreased markedly（P〈0.05）.Additionally,the level of IL-12p70 and IL-6 in serum of URSA women were significantly increased （ P〈0.01 ） .When compared with URSA patients before STD administration, the proportion of CD11c＋HLA-DR＋,CD11c＋CD80＋,CD11c＋CD86＋cells and the mRNA expression of HLA-DR,CD80,CD86 decreased＆amp;nbsp;significantly after STD administration （ P〈0.05 ） , while the mRNA and protein expression of IDO increased markedly after STD administration（P〈0.05）.Meanwhile,compared with before STD administration,serum protein level of IL-12p70 and IL-6 of URSA patients decreased significantly after STD treatment （ P〈0.01 ） .Conclusion： The changes of proportion and function of

关 键 词：原因不明性复发性自然流产 寿胎丸 树突状细胞 母胎免疫耐受 

分 类 号：R3[医药卫生—基础医学]