丙酮醛通过降低整合素β3的表达抑制内皮细胞的迁移  

Methylglyoxal inhibits human umbilical vein cell migration in vitro by down-regulating integrin β3

作　　者：庞宁波 王立群 吴剑波 

机构地区：[1]四川医科大学药物研究中心,四川泸州646000

出　　处：《南方医科大学学报》2015年第10期1395-1399,共5页Journal of Southern Medical University

基　　金：国家自然科学基金项目(81172050);四川省科技厅基金项目(2014FZ0104)~~

摘　　要：目的探讨丙酮醛对内皮细胞迁移的影响。方法浓度梯度的丙酮醛(0、25、50、100和200μmol/L)刺激体外培养人脐静脉内皮细胞(HUVECs)24 h,划痕实验和Transwell迁移实验检测丙酮醛对HUVECs迁移的影响;免疫印迹检测整合素β3的表达变化;并采用β3的抗体LM609进行干预,观察LM609对HUVECs迁移的影响。结果丙酮醛以浓度依赖的方式显著降低HUVECs的迁移(P<0.05);丙酮醛以浓度依赖和时间依赖的方式显著降低了整合素β3的表达(P<0.05);β3的特异性抗体LM609显著抑制了HUVECs的迁移(P<0.05)。结论丙酮醛通过降低整合素β3的表达抑制了HUVECs的迁移。Objective To explore the effects of methylglyoxal on endothelia cell migration. Methods Human umbilical vein endothelial cells（HUVECs） were stimulated by serial concentrations of methylglyoxal（MGO, 0, 25, 50, 100 and 200 μmol/L）for 24 h, and the cell migration was assessed by scratch wound and Transwell assay. The expression of integrin β3 in the treated cells was examined by immunoblotting, and the effect of an anti-β3 antibody, LM609, on cell migration was investigated. Results Methylglyoxal significantly inhibited HUVEC migration in a concentration-dependent manner（P〈0.05）.Methylglyoxal decreased the expression of integrin β3 in a time-and concentration-dependent manner（P〈0.05）. LM609 also significantly inhibited HUVEC migration（P〈0.05）. Conclusion Methylglyoxal inhibits HUVEC migration in vitro by downregulating integrin β3 expression.

关 键 词：丙酮醛 人脐静脉内皮细胞 整合素Β3 

分 类 号：R96[医药卫生—药理学]