三黄茵赤方含药血清对LO2细胞DNA氧化损伤的影响  

Protective effect of Sanhuangyinchi Fang drug serum on hydrogen peroxide-induced DNA oxidative damage in LO2 cells

作　　者：代欢[1] 刁建新[1] 区锦莹 李海叶[1] 杨运高[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《南方医科大学学报》2015年第10期1434-1439,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(81173262)~~

摘　　要：目的探讨三黄茵赤方含药血清对H2O2诱导LO2细胞DNA氧化损伤的保护作用。方法采集三黄茵赤方含药血清,以LO2细胞株为研究对象,通过H2O2诱导LO2细胞DNA氧化损伤模型,实验分正常对照组、H2O2模型组、三黄茵赤方含药血清5%、10%、15%浓度组、维生素E组,采用倒置显微镜观察各组形态,CCK-8法测定细胞存活率,生化法检测细胞内SOD、CAT和GSH-PX活性,流式细胞仪检测细胞内ROS水平,ELISA检测细胞内8-OHd G含量,彗星实验检测细胞DNA损伤情况。结果与H2O2模型组比较,三黄茵赤方含药血清10%、15%浓度组、维生素E组均能提高细胞存活率(P<0.05),增强细胞内SOD、CAT、GSH-PX活性(P<0.05),改善细胞对ROS清除能力(P<0.01),降低细胞内8-OHd G含量(P<0.01),减低细胞拖尾率、尾长、尾矩及Olive尾矩(P<0.05)。结论三黄茵赤方含药血清对H2O2诱导LO2细胞DNA氧化性损伤具有保护作用,以15%浓度含药血清组作用最显著。Objective To study the protective effect of Sanhuangyinchi Fang drug serum（SF） against hydrogen peroxidemediated DNA oxidative damage in LO2 cells. Methods The LO2 cells were randomly divided into the control group, H2O2 group, SF groups（5%, 10%, and 15%） and vit E group. The morphological features of the treated LO2 cells were observed under inverted microscope. The viability of the treated cells was assessed with CCK-8 method, and the activity of SOD, CAT and GSH-PX were detected biochemically. Reactive oxygen species（ROS） levels, the content of 8-OHdG, and DNA damage of the cells were evaluated by flow cytometry, ELISA, and Comet assay, respectively. Results Compared with H2O2 group, the cells in SF groups（10% and 15%） and vit E group showed higher cell survival rate（P〈0.05） and higher SOD, CAT, GSH-PX（P〈0.05） and ROS scavenging activities（P〈0.01） with markedly decreases the content of 8-OHdG（P〈0.01） and reduced tailing ratio, tail length, tail moment and Olive tail moment（P〈0.05）. Conclusion SF drug serum, especially at the concentration of15%, can protect LO2 cells from H2O2-mediated DNA oxidative damage.

关 键 词：含药血清 LO2细胞 H2O2 DNA氧化损伤 

分 类 号：R285.5[医药卫生—中药学]