稀碱-Fenton试剂预处理对云南苦竹酶水解得率的影响  

Influence of Alkaline-Fenton Pretreatment on the Enzymatic Hydrolysis Yield of Yunnan Bamboo

作　　者：王燕云[1,2] 杨静[1,2] 

机构地区：[1]西南林业大学云南省木材胶黏剂及胶合制品重点实验室,云南昆明650224 [2]西南林业大学材料工程学院,云南昆明650224

出　　处：《生物质化学工程》2015年第5期17-22,共6页Biomass Chemical Engineering

基　　金：国家自然科学基金资助项目(31260162)

摘　　要：利用响应面法对稀碱-Fenton反应预处理竹粉的条件进行优化,确定最佳的Fenton预处理条件为:1 g稀碱预处理后竹粉底物加入质量分数30%的H2O2溶液3.4 m L,Fe2+浓度15.8 mmol/L,反应时间12 h,获得的72 h酶水解得率为49.98%。与原料和经2%Na OH预处理后的样品相比,经2%Na OH-Fenton预处理后的样品中纤维素含量升高,半纤维素和木质素含量降低,72 h酶水解得率为48.24%,分别提高了47.79和37.44个百分点。当纤维素酶和β-葡萄糖苷酶的用量分别为32 FPIU/g和16 IU/g(以纤维素质量计)时,72 h酶水解得率为76.64%,比单独使用纤维素酶时的酶水解得率提高了22.80%。The optimum reaction conditions of alkaline-Fenton pretreatment of bamboo were obtained through the response surface methodology. The results showed that the optimum conditions of Fenton reaction were alkaline treated bamboo 1 g, 30% H2O2 3.4 mL, concentration of Fe^2＋ 15.8 mmol/L, and reaction time 12 h. The 72 h enzymatic hydrolysis yield was 49.98%. The sample pretreated by 2% NaOH-Fenton had higher cellulose content and lower hemicellulose and lignin content compared with the materials and sample pretreated by 2% NaOH. The 72 h enzymatic hydrolysis yield was 48.24% and increased by 47.79% and 37.44%, respectively. The 72 h enzymatic hydrolysis yield was 76.64% when the dosage of the cellulase and the β-glucosidase were 32 FPIU/g and 16 IU/g, respectively. This was 22.80% higher than that with the single use of the cellulase 32 FPIU/g.

关 键 词：响应面法 FENTON反应 酶水解得率 

分 类 号：TQ223.122[化学工程—有机化工] TQ353.1