栀子大黄汤对酒精性肝损伤保护作用的质谱评价  

Mass spectrometry assessment of the protective effects of Zhizi Dahuang decoction on alcoholic liver injury

作　　者：李欣昕[1] 陈林飞[2] 欧阳永中[2] 陈焕文[2] 冯芳[1] 

机构地区：[1]中国药科大学药物分析教研室,南京210009 [2]东华理工大学江西省质谱科学与仪器重点实验室,南昌330013

出　　处：《中国药科大学学报》2015年第5期579-586,共8页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.21225522;No.81274063;No.21265001)~~

摘　　要：采用液体辅助表面解吸常压化学电离质谱(LA-DAPCI-MS)技术对正常大鼠肝脏(正常组)、急性酒精性肝损伤大鼠肝脏(模型组)和接受栀子大黄汤治疗的肝损伤大鼠肝脏(治疗组)3组切片进行质谱成像研究,结合主成分分析(PCA)法对3组大鼠肝脏进行区分,识别出不同组动物肝内的差异性磷酸胆碱PC(34∶2),PC(36∶4)和PC(38∶4)。成像结果显示,在0.01 mm2的空间分辨率下,3种磷酸胆碱在肝组织中分布较均匀,在模型组中的含量相较于正常组和治疗组均降低,揭示了急性酒精性肝损伤与肝内磷脂变化的关系,在分子水平上评价了栀子大黄汤对急性酒精性肝损伤引起的肝内磷脂变化的保护作用。Liquid-assisted surface desorption atmospheric pressure chemical ionization mass spectrometry （LA-DAPCI-MS）was used directly for the analysis of healthy rat liver （normal control group）;acute alcoholic injury rat liver（alcohol model group）and Zhizi Dahuang decoction-treated rat liver （ZZDHD group）sections with the mass spectrometry images obtained simultaneously.Principal component analysis （PCA）was employed for the differentiation of livers of the groups;and 3 phospholipids;PC （34 ∶2）;PC（36 ∶4）and PC（38 ∶4）;were found to be the main factors.LA-DAPCI mass spectrometry images showed that the 3 phospholipids were evenly distrib-uted in liver under the spatial resolution of 0.01 mm2.Contents of 3 PCs in the model group were decreased in alcohol model group compared to the normal control group and the ZZDHD group;which revealed the relationship between acute alcoholic liver injury and intrahepatic phospholipid variation.The results showed that ZZDHD had protective effect on acute alcoholic liver injury-induced intrahepatic phospholipid variations at the molecular level.

关 键 词：栀子大黄汤 酒精性肝损伤 肝主成分分析 质谱成像 

分 类 号：R285.5[医药卫生—中药学]