瓜蒌皮提取物对大鼠血管内皮功能的影响  

Effect of EPT Against Damages of the Endothelium Induced by Low-density Lipoprotein in Rats

作　　者：谭斌[1,2] 刘思妤[1] 王桂霞[3] 曹喻灵[1] 彭军[2] 

机构地区：[1]湘南学院基础医学部药理教研室,湖南郴州423000 [2]中南大学药学院药理学系 [3]湘南学院基础医学部机能实验室

出　　处：《中南医学科学杂志》2015年第5期506-509,共4页Medical Science Journal of Central South China

基　　金：湖南省高校创新平台开放基金(12K133);湖南省中医药管理局重点项目(201227);湖南省"十二五"重点建设学科基金(湘教发[2011]76号)

摘　　要：目的研究瓜蒌皮提取物(EPT)对大鼠血管内皮功能损伤的影响,为下一步探明瓜蒌皮保护血管内皮的有效组份做准备。方法用水煎醇沉法和萃取法提取瓜蒌皮的有效组份,用不同有效组份喂养低密度脂蛋白(LDL)损伤血管内皮大鼠,并检测血液内源性相关物质。结果单次静注LDL(4 mg/kg)显著诱导血管内皮细胞损伤,增加血浆中非对称二甲基精氨酸(ADMA)水平,而降低一氧化氮(NO)水平。两种提取(70%乙醇提取和氯仿萃取)的瓜蒌皮提取物均能显著抑制LDL所致ADMA浓度升高和NO的降低。结论瓜蒌皮70%醇提取再氯仿萃取物能明显减轻血管内皮损伤,对血管有明显的保护作用。Objective To study the effect of extractive of pericarpium trichosanthis（ EPT） on damages of the endothelium induced by low-density lipoprotein（ LDL）,and find out the effective components of pericarpium trichosanthis in protecting endo-thelial cells. Methods Extract the effective component of pericarpium trichosanthis with extraction method of water decoction alcohol and precipitation. Use different effective components to feed rats of the endothelium of damages induced by LDL,and detect endogenous related substance. Results A single injection of LDL（ 4 mg/kg） significantly increased the serum levels of asymmetric dimethylarginine（ ADMA）,reduced the serum levels of nitric oxide（ NO）. EPT with the different abstraction（ 70% alcohol and chloroform） markedly reduced the level of ADMA,,and increased the level of NO. Conclusion EPT with the different abstraction（ 70% alcohol and chloroform） protects the endothelium against damages induced by LDL.

关 键 词：瓜蒌皮提取物 血管内皮 功能 

分 类 号：R96[医药卫生—药理学]