基于PLSR分析当归挥发油分子蒸馏馏分中化学成分与抗炎作用的相关性  

Correlation between chemical constituents and anti-inflammatory activity of distillates separated from essential oil of Angelica Sinensis Radix by molecular distillation based on PLSR

作　　者：张庆[1] 茹庆国 林红梅[1] 刘艳[1] 康倩[1] 李辉[1] 吴清[1] 

机构地区：[1]北京中医药大学中药学院,硕士研究生100102

出　　处：《环球中医药》2015年第10期1153-1158,共6页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81073059);北京中医药大学创新团队发展计划(2011-CXTD-13)

摘　　要：目的研究当归挥发油分子蒸馏馏分的化学成分与其抗炎作用的相关性。方法用分子蒸馏设备对当归挥发油进行分馏,气质联用色谱(gas chromatography-mass spectroscopy,GC-MS)表征所得馏分的化学组成;用脂多糖(lipopolysaccharide,LPS)诱导RAW264.7细胞作为炎症模型,评价各馏分的细胞毒作用和抗炎作用;用偏最小二乘法回归分析(partial least squares regression,PLSR)分析馏分中化学成分与抗炎作用的相关性。结果当归挥发油经分子蒸馏后得到6个馏分,且各馏分均能抑制LPS诱导的RAW264.7细胞产生一氧化氮,表现出抗炎作用;PLSR结果显示建立的回归模型合理,馏分中的十九烷、2,2-二甲基-1-苯基-1-丙醇、Z-藁本内酯、E-藁本内酯、十六烷、阿魏酸、十三酸、亚油酸、油酸、5,8,11-十七碳三炔酸甲酯与抗炎作用呈正相关。结论本研究为当归及其他中药挥发油的进一步开发利用及中药挥发油的质量控制提供了实验数据。Objective To investigate the correlation between the chemical constituents and anti-inflammatory effect of distillates separated from the essential oil of Angelica Sinensis Radix by molecular distillation. Methods The distillates were separated from the essential oil of Angelica Sinensis Radix by molecular distillation, and the chemical constituents of distillates were analyzed by GC-MS;lipopolysaccharide （LPS） - induced RAW264. 7 cells as a model of inflammation to evaluate the cell toxicity and anti-inflammatory effects of distillates;partial least squares regression （ PLSR） was evaluated to analysis the correlation between chemical constituents and anti-inflammatory effect. Results 6 fractions obtained from Angelica Sinensis Radix by molecular distillation,and all distillates showed anti-inflammatory effects by inhibiting the production of NO by LPS activated RAW264. 7 cells;PLSR results showed that the regression model was reasonable, and Nonadecane, Benzenemethanol, a-（ 1, 1-dimethylethyl ）-, Z-ligustilide,E-ligustilide, Hexadecane, ferulaic acid, tridecanoic acid, linoleic acid, oleic acid, 5, 8, 11-Heptadecatriynoic acid, methyl ester were positively related to anti-inflammatory effects. Conclusion This study provided the experimental data for the further development of the essential oil of Angelica Sinensis Radix and other Chinese herbs and the quality control of essential oil of Chinese medicine.

关 键 词：当归挥发油 分子蒸馏 气质联用色谱 抗炎 偏最小二乘法 

分 类 号：R285.5[医药卫生—中药学]