均匀设计法优选掌叶大黄(Rheum palmatum L.)总蒽醌类物质的薄层层析展开剂体系  

On the Developing Solvent System of Thin-layer Chromatography through Uniform Design Method in Extracting the Total Anthraquinones Constituents from Rheum Palmatum L

作　　者：井明博[1,2] 段春燕[1,2] 肖朝霞[1,2] 焦飞[1,2] 李东波[1,2] 卜婷[1,2] 贾海燕[1,2] 毛宁[1,2] 

机构地区：[1]陇东学院生命科学与技术学院 [2]甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃庆阳745000

出　　处：《陇东学院学报》2015年第5期66-69,共4页Journal of Longdong University

基　　金：甘肃省庆阳市重大科技支撑计划项目(1003NKCM020)

摘　　要：以陇东地区子午岭掌叶大黄为实验材料,采用均匀设计法设计试验,以各成分的Rf值为评价指标,分析比较了各展开体系对掌叶大黄总蒽醌类成分的展开情况。结果表明:基于均匀设计法进行的12种展开系统中,以丙酮∶甲醇∶正己烷∶1,2-二氯乙烷=1∶1∶1∶1展开体系效果最佳,展开斑点个数达9个,且各Rf值间差异极显著(P<0.01)。本研究采用均匀设计法优选了大黄总蒽醌类成分的薄层层析展开剂,与药典记录的游离蒽醌展开剂相比,具有样品预处理简单、节约有机溶剂等优越性,可为大孔吸附树脂法进一步分离蒽醌类物质的定性分析提供数据参考。Taking the-Rheum palmatclm collected from the Ziwuling region as the experimental materials and taking the developing situation （Rf value） of its total anthraquinones constituents into consideration, analyzes the different developing solvent systems through uniform design method. The results show that the optimal developing solvent systems are as follows： acetone to methanol to n-hexane to 1,2-dichloro- ethane equals 1 ： 1 ： 1 ： 1, which could demonstrate 9 different spots on the chromatoplate, and their Rf val- ues are different significantly （ P 〈 0.01 ）. Compared with developing solvent systems recorded in the Pharmacopoeia of the People＇ s Republic of China, the optimal the total anthraquinones substances develo- ping solvent system of thin-layer chromatography is selected in this study, and it demonstrates the advanta- ges including simple sample pretreatment and economizing organic solvent, which would offer the refer- ence data for further study on qualitative analysis of anthraquinone compounds by macroporous adsorption resin separation.

关 键 词：掌叶大黄 总蒽醌 均匀设计法 薄层层析法 展开剂 

分 类 号：R932[医药卫生—生药学]