中脑腹侧被盖区参与大鼠慢性内脏痛的调节  

THE VENTRAL TEGMENTAL AREA IS INVOLVED IN THE REGULATION OF CHRONIC VISCERAL PAIN IN RATS

作　　者：康洁[1] 赵冰雪[1] 邵博明 张咏梅[1] 

机构地区：[1]江苏徐州医学院麻醉学重点实验室,徐州221000

出　　处：《中国疼痛医学杂志》2015年第10期745-749,共5页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助项目;No.81171041;江苏省高校自然科学研究重大基金资助项目;No.13KJA320001

摘　　要：目的:探讨中脑腹侧被盖区在慢性内脏痛调节中的作用。方法:新生期8、10和12天的大鼠进行结直肠扩张刺激来制备慢性内脏痛模型;采用免疫荧光法检测中脑腹侧被盖区内c-fos表达情况和小胶质细胞活化情况;通过利多卡因微量注射中脑腹侧被盖区后观察内脏痛行为学和c-fos的变化情况。结果:与对照组相比:(1)模型大鼠痛阈值降低,腹外斜肌放电和腹壁撤退反射评分显著增加(P<0.05),表明内脏痛模型成功建立;(2)中脑腹侧被盖区c-fos表达增加,神经元兴奋性增高,注射0.5μl 4%利多卡因后,腹壁撤退反射评分降低,痛阈值增高(P<0.05),并且c-fos表达减少;(3)内脏痛模型大鼠小胶质细胞活化,Iba-1表达增多(P<0.05)。结论:中脑腹侧被盖区参与大鼠慢性内脏痛的调节。Objective： To investigate the effect of the ventral tegmental area on the regulation of chronic visceral pain in rats. Methods： The chronic visceral pain model was induced by colorectal distension（CRD） on rats 8, 10 and 12 days after birth. The expression of c-fos and activation of microglia in ventral tegmental area were detected by immunofluorescent staining. The changes of visceral pain behavior and c-fos expression were also observed before and after injection of lidocaine into the ventral tegmental area. Results： Compared with control group：（1） the distention threshold decreased, and the abdominal withdrawal reflex scores and external oblique discharge amplitude increased significantly in CRD group rats（P〈0.05）;（2） the expression of c-fos increased in CRD group. After microinjection of lidocaine into the ventral tegmental area, abdominal withdrawal reflex scores significantly decreased and distention threshold increased（P〈0.05）. Moreover, the c-fos expression was inhibited by lidocaine（P〈0.05）;（3） the microglia were activated and the Iba-1 expression increased in CRD group（P〈0.05）. Conclusion： The ventral tegmental area is involved in the formation of chronic visceral pain in rats.

关 键 词：结直肠扩张刺激 中脑腹侧被盖区 内脏痛 C-FOS 小胶质细胞 

分 类 号：R402[医药卫生—临床医学]