骨髓间充质干细胞在大鼠脊髓损伤治疗中的应用  

Application of Bone Marrow Mesenchymal Stem Cells in Treatment of Rats with Spinal Cord Injuries

作　　者：薛荣利[1] 张媛媛[1] 李泽慧[1] 周丹[1] 张晨晨[1] 哈小琴[1] 

机构地区：[1]兰州军区兰州总医院检验中心甘肃省干细胞与基因药物重点实验室,兰州730050

出　　处：《解放军医药杂志》2015年第10期4-7,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：全军"十二五"面上项目(CWS11C229)

摘　　要：目的探讨骨髓间充质干细胞(MSCs)移植对大鼠脊髓损伤(SCI)修复的作用。方法全骨髓贴壁法分离培养骨髓MSCs,采用自由落体撞击法制作大鼠SCI模型,建模大鼠存活24只,分为移植组和对照组,每组12只,移植组在损伤部位移植培养的骨髓MSCs,对照组给予等量生理盐水。根据联合行为学评分评价两组大鼠运动能力恢复情况,观察脊髓组织病理学变化、神经细胞损伤与修复及SRY基因的表达情况。结果移植组治疗后不同时间联合行为学评分均高于对照组(P<0.05)。对照组脊髓组织中出现较多空腔,神经纤维及细胞坏死;移植组脊髓组织空泡减少,增生组织增多,神经纤维及细胞增多,且有SRY基因的表达。结论骨髓MSCs移植可促进大鼠SCI修复。Objective To investigate the effect of transplanting bone mesenchymal stem cells（ MSCs） in repair of rats with spinal cord injuries（ SCI）. Methods Bone marrow MSCs were isolated and cultured using whole bone marrow cells adherence method,and models of SCI were established using free falling body hitting method. A total of 24 rats survived after model establishment,and were divided into transplantation group（ n = 12） and control group（ n = 12）.Transplantation group was transplanted with mesenchymal stem cells in injury sites,while control group was treated with same values of physiological saline. The recovery conditions of locomotory capabilities were evaluated using combined praxiology score,and the medullary histopathological changes,injuries and repair of nerve cells and expression of SRY gene were observed. Results The combined praxiology scores of transplantation group at different time points after the treatment were higher than those of control group（ P 0. 05）. In control group,there were cavities in spinal cord tissues and dead nerve fibers and cells. In transplantation group,the number of cavities was decreased,but hyperplastic tissues was increased uncreased as well as nerve fibers and cells,and SRY gene expression was found. Conclusion Transplantation of bone marrow MSCs may improve SCI repair.

关 键 词：骨髓细胞 间质干细胞移植 脊髓损伤 大鼠 

分 类 号：R651.21[医药卫生—外科学] R332[医药卫生—临床医学]