Box-Behnken响应面设计法优化微波辅助提取猪苓多糖工艺  

Microwave-Assisted Extraction of Polysaccharides from Polyporus umbellatus Optimized by Box-Behnken Design-Response Surface Methodology

作　　者：乔艳明[1] 陈文强[1,2] 邓百万[1,2] 彭浩[1,2] 解修超[1,2] 张辉 潘嘉伟 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723000 [2]陕西省食药用菌工程技术研究中心,陕西汉中723000 [3]陕西省南郑中学,陕西南郑723100

出　　处：《食品与生物技术学报》2015年第9期986-994,共9页Journal of Food Science and Biotechnology

基　　金：陕西省"春笋计划"研究项目(2014-2015)

摘　　要：为了优化猪苓多糖的微波提取工艺,采用Box-Behnken响应面设计法,研究液料比、p H、微波功率、提取时间、提取次数及其交互作用对多糖提取率的影响。应用Design Expert和响应面分析相结合的方法,模拟得到回归方程的预测模型和可信度,分析得到最佳提取工艺条件为:液料比30∶1(m L/g),p H 6.6,微波功率614 W,提取时间2.5 min,提取次数2次。在此条件下,多糖提取率达到6.75%。利用Box-Behnken响应面设计法优化得到的猪苓多糖提取条件参数,为猪苓多糖工业化生产提供技术支持。To optimize the microwave-assisted extraction technique of polysaccharide from Polyporus umbellatus,the effects of the liquid to solid ratio,p H,microwave power,extraction time,extraction number and their interaction on extaction were studied by Box-Behnken design. The predictive model and reliability were developed by Design Expert software and response surface analysis（RSM）. The optimal extraction conditions were achieved and listed as follows：liquid-to-solid ratio 30 ∶1 m L/g,p H 6.6,microwave power 614 W,extraction time 2.5 mins,extraction number 3 times. Under these conditions,the extraction yield of polysaccharide was up to6.75%. The extraction conditions achieved by RSM provided technical support for the industrial production of polysaccharide from Polyporus umbellatus.

关 键 词：猪苓 微波辅助提取 多糖 BOX-BEHNKEN设计 响应面分析 

分 类 号：TQ464.1[化学工程—制药化工]