血小板贮存方法对血小板活化分子CD62p表达的影响  

Effect of different storage methods on expression of platelet activation marker CD62p

作　　者：刘更夫[1] 黄飞[2] 梅方超[1] 周庆申[3] 

机构地区：[1]黄石市中心医院输血科,湖北黄石435000 [2]湖北理工学院医学院 [3]黄石市中心血站

出　　处：《临床血液学杂志（输血与检验）》2015年第5期832-834,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的：探讨常温贮存及冰冻保存血小板对血小板活化分子CD62p表达的影响。方法：选择2013-06-2014-05符合无偿捐献血小板标准的献血者血小板85份,采用流式细胞术检测常温贮存0、1、3、5d和冰冻贮存1、2、3、5个月血小板表面活化分子CD62p表达率,ELISA方法检测新鲜血小板可溶性CD62p（sCD62p）的表达水平,比较不同贮存方法和贮存时间对血小板活化的影响。结果：常温贮存组CD62p表达率和sCD62p水平随着贮存时间的延长而升高（P〈0.05）,冰冻保存对血小板活化分子CD62p表达率有影响（P〈0.05）,冰冻保存不同时长对CD62p表达率没有影响（P〉0.05）。结论：常温贮存血小板活化分子CD62p活化率随着贮存时间延续而升高,贮存时间0～1d的适用于以提高血小板计数的患者,贮存时间长的新鲜和冰冻保存血小板高适用于急性出血患者。Objective：To study the effect of different storage on the expression of platelet activation marker CD62 p.Method：A total of 85 platelets concentrates were obtained from healthy donors by Amicus separator from Jun 2013 to May 2014.The expression of CD62 p on platelet from fresh platelet（0,1,3,and 5days）and from cryopreserved platelet（1,2,3and 5months）were detected by flow cytometry successively.The soluable sCD62 p was analyzed by ELISA.Result：CD62p expression and soluable sCD62 p in fresh platelet increased along with the storage time（P〈0.05）.CD62 p expression was higher in cryopreserved platelet compared with in fresh platelet（P〈0.05）.The level of CD62 p expression in cryopreserved platelet did not change following the storage time（P〉0.05）,which was related with the methods of platelets storage.CD62 p expression and the levels of sCD62 p were on the rise with time extending of the storage of fresh platelet（P〈0.05）.CD62 p expression and the levels of sCD62 p of the short time storage of platelets were significant lower than that of one of other storage methods of platelets.Conclusion：CD62p expressions of fresh platelet with the short time storage were less than that of cryopreserved platelet with the long time storage.Cryopreserved platelet with the long time storage could apply to acute hemorrhagic disease.

关 键 词：血小板 活化分子 CD62P 可溶性CD62p 

分 类 号：R331.1[医药卫生—人体生理学]