盐碱胁迫下金叶接骨木根系K含量模型构建"  

Establishment of The Estimation Model of K Content of Sambucus canadensis Root under Saline-Alkali Stress

作　　者：石慧勤 张罗沙 邸葆[3] 李保会[2] 张芹[1] 

机构地区：[1]河北农业大学园林与旅游学院,保定071000 [2]河北农业大学林学院,保定071000 [3]河北农业大学园艺学院,保定071000

出　　处：《林业科学》2015年第9期18-23,共6页Scientia Silvae Sinicae

基　　金：国家星火计划项目(2012GA105005);河北省林业厅项目(1404452)

摘　　要：【目的】以金叶接骨木为试验材料,用电阻抗图谱(EIS)法和电解质渗透率法测定根系抗盐碱性并构建其根系K含量模型,为简单快速地预测盐碱胁迫下金叶接骨木根系的K含量提供技术支持。【方法】研究不同浓度盐碱胁迫下金叶接骨木根系EIS图谱、细胞膜透性以及K含量的变化,将根系EIS参数与相应的K含量进行线性和非线性回归分析,选取最佳的EIS参数构建估测模型,并对模型进行精度检验。【结果】在胁迫处理期间,各浓度处理金叶接骨木根系K含量均显著低于对照(P≤0.05);随着盐碱胁迫时间延长,各浓度处理下金叶接骨木根系的相对电导率呈现上升趋势,且均显著高于对照(P≤0.05);随着盐碱胁迫时间延长,各浓度处理下金叶接骨木根系的电阻抗图谱变化不同;弛豫时间τ为估测金叶接骨木根系K含量的最佳EIS参数,最佳估测模型为y=0.005 5x3-0.232 8x2+2.638 8+15.877,预测精度达到91.73%。【结论】利用EIS参数构建的金叶接骨木根系K含量模型y=0.005 5x3-0.232 8x2+2.638 8x+15.877预测精度高,可以用于快速准确预测盐碱胁迫下金叶接骨木根系K含量的变化。[Objective]Sambucus canadensis was used as experimental material in this study. To simply and fastly predict K content in roots of Sambucus canadensis under salinity and alkalinity stress,salinity and alkalinity resistance of this plant roots was measured by electrical impedance spectroscopy （ EIS ） and electrolyte permeability methods and the estimation model of K Content of their roots was establish. [Method]The electrical impedance spectroscopy, cell membrane permeability and the change of K content in roots during different concentrations of salinity-alkalinity stress were studied. The corrections between EIS parameters and K content were analyzed using linear and nonlinear regressions. The estimation model was constructed using the best EIS parameter and the model accuracy was tested.[Result]During the salinity and alkalinity stress,compared with control,the K content of Sambucus canadensis roots treated by different concentrations of salinity-alkalinity significantly decreased（P≤0. 05）. As the extension of salinity-alkalinity stress time, the relative conductivity of the roots under different stress tended to rise and significantly increased compared with control （ P≤0. 05 ） . The spectra of electrical impedance changed in roots of Sambucus canadensis during salinity-alkalinity stress;The EIS parameter τ was the best for assessing K content of root. The best model was y =0. 005 5x3 -0. 232 8x2 ＋2. 638 8x ＋ 15. 877 with the precision accuracy of 91. 73%.[Conclusion]The model y =0. 005 5x3 -0. 232 8x2 ＋2. 638 8 x＋ 15. 877 with best precision accuracy constructed with EIS parameter τ can be used to fastly and accurately forecast K content in roots of Sambucus canadensis under salinity and alkalinity stress.

关 键 词：金叶接骨木 根系 盐碱胁迫 电阻抗图谱参数 K含量 相对电导率 模型构建 

分 类 号：S158.3[农业科学—土壤学]