基质和IBA浓度对云锦杜鹃扦插生根的影响  

Effects of Media and IBA Concentrations on Rooting of Rhododendron fortunei for Cutting Propagation

作　　者：王书胜[1] 单文[1] 张乐华[1] 杜有新[1] 李晓花[1] 王凯红[1] 

机构地区：[1]江西省中国科学院庐山植物园,庐山332900

出　　处：《林业科学》2015年第9期165-172,共8页Scientia Silvae Sinicae

基　　金：国家国际科技合作项目(2014DFA31720;2007DFA31410);"十二五"国家科技支撑计划课题(2011BAC13B01;2011BAC13B04);"赣鄱英才555工程"领军人才培养计划项目;江西省学科带头人培养计划项目(2010DD00500)

摘　　要：【目的】基质组成与外源激素应用是影响扦插生根的重要因子。云锦杜鹃扦插生根困难,本研究旨在探讨不同基质、IBA浓度及其交互效应对该物种扦插生根的影响,筛选出最佳扦插基质与IBA浓度组合,为引种驯化与产业化开发提供理论依据。【方法】以云锦杜鹃当年生带芽苞的木质化顶枝为材料,采用纯河沙、泥炭+珍珠岩(4∶1,体积比)、珍珠岩+森林土(4∶1)、蛭石+森林土(4∶1)、河沙+森林土(1∶1)、纯森林土6种扦插基质与100,200,400,800 mg·L-14个IBA浓度的两因素完全随机区组设计,研究不同基质与IBA浓度处理组合对云锦杜鹃插穗愈伤组织诱导率、腐烂率、生根率、老叶留存率及总根数、不定根数、最长不定根长、根幅等生根指标的影响,并运用隶属函数法对各处理组合的生根效果进行综合评价。【结果】扦插基质、IBA浓度对所有测试指标均有极显著的(P<0.01)影响,且基质的影响大于IBA浓度;两者交互效应仅对总根数、不定根数、最长不定根长及根幅4个根系发育指标有极显著的影响。6种基质中,纯河沙除最长不定根长、根幅表现较差外,其他指标表现极佳;泥炭+珍珠岩(4∶1)在愈伤率、最长不定根长及根幅上表现最佳,其他指标仅次于纯河沙;其他4种基质的生根效果随着森林土配比增加而下降,纯森林土在所有指标上表现最差。4个IBA浓度上,愈伤率、腐烂率以100 mg·L-1处理最佳,随着IBA浓度升高愈伤率显著降低、腐烂率升高;生根率、老叶率以200 mg·L-1处理最佳,随着IBA浓度进一步升高而逐渐降低;总根数、不定根数、最长不定根长及根幅在100,200,400 mg·L-1浓度间无显著差异,但均显著优于800 mg·L-1处理。隶属函数评价表明,24个处理组合中纯河沙与200 mg·L-1IBA组合为云锦杜鹃扦插生根的最佳组合,其腐烂率、生根率、老叶率、总根数及不定根数在所有组合中表现最�[Objective]Medium composition and application of exogenous hormones are important factors that influence the rooting of cuttings. As it is hard to root for cutting propagation of Rhododendron fortunei,this study was aimed at investigating different media,indole-3-butyric acid （ IBA） concentrations and their interactions on the rooting of cuttings of this species,and to select the optimum combination of rooting medium and IBA concentration,then to provide a theoretical basis for its introduction and domestication,and industrialization. [Method]Using the lignified new terminal branches with leaf buds of R. fortunei as propagating material,and adopting a completely randomized factorial design with two factors,i. e. ,six types of rooting media： pure river sand,peat ＋ perlite （4∶1 by volume）,perlite ＋ forest soil （4∶1）,vermiculite ＋ forest soil （ 4∶ 1 ）,river sand ＋ forest soil （ 1∶ 1 ） and pure forest soil,and four IBA＆amp;nbsp;concentrations：100 ,200 ,400 ,800 mg·L -1 ,we investigated the effects of different combinations of rooting medium and IBA concentration on rooting parameters of R. fortunei,i. e. percentages of callus formation,rotting rate,rooting rate, and retaining rate of old leaves,number of total roots and number of adventitious roots,length of longest adventitious root, width of root system. And then we comprehensively evaluated the effects on rooting of each treatment combination by subordinate function analysis. [Result]The results showed that the effects of rooting medium and IBA concentration were highly significant （P〈0. 01） for all measured parameters,and the influencing intensity of rooting medium was stronger than that of IBA concentration. The interaction of rooting medium and IBA concentration was highly significant only for four root development parameters including the number of total roots,number of adventitious roots,length of the longest adventitious root and root width. Among the six types of rooting media,the pure river sand medium produ

关 键 词：云锦杜鹃 扦插繁殖 基质 IBA浓度 生根能力 根系发育 

分 类 号：S723.13[农业科学—林木遗传育种]