拟南芥AtSb10基因的克隆及其过表达转基因株系的获得  

Cloning of AtSb10 gene in Arabidopsis thaliana and over-expression of AtSb10 in Arabidopsis thaliana

作　　者：赵莺婕[1] 刘春林[2] 阮颖[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南农业大学农学院,湖南长沙410128

出　　处：《浙江农业学报》2015年第9期1550-1554,共5页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31071455)

摘　　要：拟南芥At Sb10基因是一个表达谱和功能都未知的基因,生物信息学分析发现其氨基酸序列含有2个连续的MATH结构域。为分析At Sb10基因的功能,首先,利用RT-PCR方法检测At Sb10基因在拟南芥中的表达模式,结果表明,该基因只在根中表达,而茎、叶片、花与果荚中未检测到;然后克隆了At Sb10基因全长,构建了过量表达载体,通过农杆菌介导法将该载体转入拟南芥Col-0中,获得了10株过表达转基因植株,为进一步研究At Sb10基因的功能奠定了基础。Bioinformatic analysis suggested that At Sb10,whose function and expression profile were unknown,contained two consecutive MATH domain structures. In order to further explore the function of At Sb10 gene,RT-PCR was used to investigate the expression profile of Atsb10 in Arabidopsis thaliana. The results showed At Sb10 gene was expressed only in roots,not in stems,leaves,flowers and siliques. Meanwhile,full-length of At Sb10 was cloned into overexpression binary vector p CAMBIA1300 driven by the Ca MV35 S promoter. Then this expression vector was transformed into Columbia-0 ecotype mediated by Agrobacterium tumefaciens GV3101. Ten over-expression transgenic plants were obtained,which would provide good materials for further studying on the function of Atsb10.

关 键 词：拟南芥 AtSb10 生物信息学分析 表达谱 

分 类 号：S637[农业科学—蔬菜学] Q943.2[农业科学—园艺学]