TCDD对建鲤肝脏的影响  

Effect of TCDD on fish liver in Jian carp( Cyprinus carpio var. Jian)

作　　者：杜金梁[1,2] 曹丽萍[1,2] 刘英娟[3] 赵才源[3] 申玉金 丁炜东[1,2] 殷国俊[1,2,3] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心,农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业部鱼类免疫药理学国际联合实验室,江苏无锡214081 [3]南京农业大学无锡渔业学院,江苏无锡214081

出　　处：《浙江农业学报》2015年第10期1720-1724,共5页Acta Agriculturae Zhejiangensis

基　　金：国家自然基金青年科学基金(31202002);国家自然基金青年科学基金(31200918);中央级公益性科研院所基本科研业务费专项资金(2013JBFM12);中国水产科学研究中央级公益性科研院所基本科研业务费专项资金项目(2014A08 YQ01)

摘　　要：研究2,3,7,8-四氯二苯并-p-二噁英(TCDD)对建鲤肝组织的影响。将建鲤随机分为5个处理组和1个空白对照组,每组30尾鱼。采用一次性腹腔注射染毒,处理组注射剂量分别为0.1,0.3,0.6,1.2,2.4μg·kg-1,空白组注射同体积二甲基亚砜(DMSO)。分别于给药后24,48,72,96,120 h采集血液及肝组织,测定谷草转氨酶(GOT)、谷丙转氨酶(GPT)、乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽S-转移酶(GST)活力和总抗氧化能力(T-AOC)的变化。结果表明:TCDD染毒72 h,各染毒组损伤程度较严重,GOT,LDH,GPT活性值显著升高(P<0.01或P<0.05),GST,GSH-Px,T-AOC活力显著降低(P<0.01或P<0.05)。这说明TCDD急性染毒后可以造成建鲤肝组织的损伤,引起建鲤肝组织的氧化应激反应。In order to evaluate the toxic effect of TCDD on fish liver,Jian carp were randomly divided into 6 groups（ 30 fish for each group） including a control group. The treated fish were injected with TCDD（ 0. 1,0. 3,0. 6,1. 2,2. 4 μg·kg- 1,respectively）. The control group had no TCDD injection,but was injected the same volume of DMSO. The blood and fish liver were collected at 24,48,72,96,120 h after injection,respectively,and the expression levels of LDH,GPT,GOT in Jian carp serum and GSH-Px,GST,T-AOC activity in liver homogenates were determined. It was shown that,after injection with TCDD for 72 h,GOT,GPT,LDH activities were significantly increased（ P〈0. 01 or P〈0. 05）,while GST,GSH-Px,T-AOC activities were significantly decreased（ P〈0. 01 or P〈0. 05）,indicating that TCDD could induce serious damage on fish liver and cause oxidative stress reaction inJian carp after acute exposure.

关 键 词：2 3 7 8-四氯二苯-p-二噁英 谷草转氨酶 谷丙转氨酶 乳酸脱氢酶 谷胱甘肽过氧化物酶 谷胱甘肽S-转移酶 总抗氧化能力 

分 类 号：S948[农业科学—水产养殖] X174[农业科学—水产科学]