利用SSR技术研究浙江省稻瘟病菌群体的遗传多样性  

Analysis of genetic diversity of Magnaporthe oryzae isolates from Zhejiang Province based on SSR markers

作　　者：谢晶晶[1,2] 姜华[2] 毛雪琴[2] 柴荣耀[2] 邱海萍[2] 张震[2] 王艳丽[2] 王教瑜[2] 杜新法[2] 孙国昌[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省植物有害生物防控重点实验室,省部共建国家重点实验室培育基地,浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2015年第10期1781-1788,共8页Acta Agriculturae Zhejiangensis

基　　金：浙江省公益技术研究农业项目(2013C32019,2012C22024);浙江省自然科学基金(Y3110537);农业部公益性行业项目(201203014)

摘　　要：为研究浙江省稻瘟病菌群体的遗传多样性,利用分布于稻瘟病菌7条染色体上的28对SSR引物,对浙江省11个城市1978至2011年11个城市的稻瘟病菌的遗传结构多样性进行了分析。结果表明,28对SSR引物共扩增出171个等位基因,每对引物可扩出2～24个等位基因,平均多样性指数0.534,平均多态性信息含量为0.494。分离于不同地理位置的稻瘟病菌株的多样性较高,其中丽水和杭州的遗传多样性最高,舟山的遗传多样性最低。分离于不同年份的稻瘟病菌的遗传结构差异较大,1990年的遗传多样最高,1978年的遗传多样性最低。分离于不同水稻类型的稻瘟病菌的遗传多样性较高,部分分离于籼稻和粳稻的稻瘟病菌的遗传结构相似甚至相同,分离于籼稻的稻瘟病菌的遗传多样性高于分离于粳稻的稻瘟病菌。Twenty-eight pairs of SSR primers that distributed on 7 chromosomes of Magnaporthe oryzae were used to study the genetic diversity of M. oryzae isolates from Zhejiang Province. M. oryzae strains isolated from 11 different areas in Zhejiang Province,from 1978 to 2011 were used as materials. Results showed that the total amplified alleles were 171,the amplified alleles of each primer pairs was 2 to 24,the average diversity index was 0. 534 and the average polymorphism information content was 0. 494. The genetic structure of M. oryzae strains isolated from different areas was different,Lishui and Hangzhou populations had the highest genetic diversity and Zhoushan population had the lowest genetic diversity. Genetic diversities of M. oryzae isolates from different years were different,the 1990 population had the highest genetic diversity,while the 1978 population had the lowest genetic diversity. The genetic structure of M. oryzae isolated from different rice types was different,a part of strains isolated from indica and japonica had the similarity genetic structure,and the strains isolated from indica rice had the higher genetic diversity than those isolated from japonica rice.

关 键 词：稻瘟病菌 SSR 遗传结构 多样性 

分 类 号：S432.44[农业科学—植物病理学]