雷蒙德氏棉DnaJ蛋白的生物信息学预测及盐胁迫下的表达分析  

Genome-wide Identification and Expression Pattern Analysis of the Dna J Protein Family in Gossypium raimondii under Salt Stress Using a Bioinformatics Method

作　　者：徐珍珍[1] 郭琪[1] 刘静[2] 徐鹏[1] 张香桂[1] 沈新莲[1] 

机构地区：[1]江苏省农业科学院经济作物研究所/农业部长江下游棉花和油菜重点实验室,南京210014 [2]江苏省农业科学院生物技术研究所/江苏省农业生物学重点实验室,南京210014

出　　处：《棉花学报》2015年第5期391-400,共10页Cotton Science

基　　金：江苏省农业科技自主创新[CX(14)5008];棉花生物学国家重点实验室开放课题(CB2014A01);国家科技重大专项"转基因生物新品种培育"(2014ZX08005-004-002);江苏省自主创新基金[CX(14)2065]

摘　　要：Dna J蛋白是一类很大的蛋白家族,是近年来研究较多的与胁迫反应等相关的蛋白。本文利用生物信息学手段,系统研究了雷蒙德氏棉(Gossypium raimondii)Dna J蛋白的数目、分类、染色体定位、亚细胞定位、进化以及在旱地棉(Gossypium aridum)和克劳茨基棉(G.klotzschianum)盐胁迫下的表达模式。结果显示:雷蒙德氏棉基因组有119个Dna J蛋白,长度从73个氨基酸到2574个氨基酸不等;在13条染色体上不均匀分布,且具有不同的亚细胞定位;Dna J结构域大致被分为9个小组,每个小组都有与拟南芥同源的基因;盐胁迫下,鉴定到旱地棉和克劳茨基棉共同差异表达的Dna J基因3个,在旱地棉盐胁迫整个过程中差异表达的基因2个。雷蒙德氏棉Dna J基因家族的鉴定和分析,将为进一步研究Dna J基因家族的功能奠定一定的基础。Dna J proteins comprise a large family and have been shown to be involved in various stress responses in recent studies.In this study, we systematically studied the number, types, chromosome distribution, subcellular localization and evolutionary relationships of Dna J proteins in the Gossypium raimondii using a bioinformatics method. In parallel, we studied the expression patterns of Dna J proteins in G. aridum and G. klotzschianum under salt stress. 119 Dna J family members were identified with an uneven chromosome distribution and various subcellular localizations. The amino acid lengths of the Dna J protein family members varied from 73 to 2574. Their Dna J domains could be divided into nine groups, and there were homologs of Arabidopsis thaliana genes in each group. Three Dna J gene family members were differentially expressed under salt stress in both G. aridum and G. klotzschianum, and two genes were differentially expressed at every time point under salt stress in G. aridum. The identification and bioinformatics analysis of the Dna J gene family in G. raimondii will provide a firm foundation for future study of these genes＇ functions in cotton.

关 键 词：Dna J蛋白 雷蒙德氏棉 旱地棉 克劳茨基棉 生物信息学 表达模式 差异表达基因 

分 类 号：S562[农业科学—作物学]