HH胶囊治疗HBV转基因小鼠抗乙肝病毒研究  

Anti- Hepatitis B Virus Research on HH capsule Treating HBV Transgenic Mice

作　　者：张传涛[1] 张莉敏[1] 黄群[2] 杨鸿[1] 郭尹玲[2] 王芳瑜[2] 

机构地区：[1]成都中医药大学附属医院,四川成都610075 [2]成都中医药大学,四川成都610072

出　　处：《时珍国医国药》2015年第10期2314-2315,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81202624;No.81102720;No.81072902)

摘　　要：目的建立HH胶囊的高效液相色谱(HPLC)指纹图谱,并观察HH胶囊的抗乙肝病毒(Hepatitis B virus,HBV)疗效。方法采用大连依利特ODS-BP C18色谱柱对HH胶囊的HPLC指纹图谱研究。HBV转基因小鼠随机分4组,苦参素组小鼠给予同体积含150mg/(kg·d)苦参素混悬液,高、低剂量HH胶囊组小鼠分别给予同体积含7,2g/(kg·d)HH混悬液,5周后,Elisa法检测血清及肝组织HBs Ag,荧光定量PCR法检测血清及肝组织HBVDNA。结果标定14个特征峰构成HH胶囊的指纹图谱,确定其中1号峰为没食子酸、8号峰为柯里拉京、9号峰为虎杖苷、10号峰为鞣花酸、13号峰为甘草酸、14号峰为齐墩果酸。高剂量HH胶囊[7g/(kg·d)]可以显著降低HBV转基因小鼠肝脏和血清中的HBs Ag、HBVDNA水平,差异具有统计学意义(P<0.01、P<0.05),与苦参素组比较差异无统计学意义(P>0.05)。结论 HH胶囊具有确切的抗HBV作用,HPLC法对HH胶囊质量控制可靠。Objective To establish HPLC fingerprint of HH capsule and observe the anti- HBV efficacy of HH capsules. Methods Dalian Elite ODS- BP C18 column was used to study the HH Capsules HPLC fingerprint. HBV transgenic mice were randomly divided into four groups,Matrine group was fed the same volume suspension containing 150 mg / kgod Matrine,high and low dose HH capsule groups were given the same volume suspension containing 7g /（ kg·d）,2g /（ kg·d） HH. After treatment for five weeks. Elisa method was used to detect HBs Ag in the serum and liver tissue,quantitative PCR was used to detect HBVDNA in serum and liver tissue. Results The final calibrated 14 characteristic peaks constituted HH capsules fingerprint,the 1st peak was Gallic acid,the 8th peak Corilagin,the 9th peak Polydatin,the 10 th peak Ellagic acid,the 13 th peak Glycyrrhizin,the 14 th peak Oleanolic acid. High dose [7g /（ kg·d） ] HH capsule could significantly reduce HBs Ag,HBVDNA in the serum and liver of HBV transgenic mice,the difference was statistically significant（ P〈0. 01, P〈0. 05）,compared with Matrine group,the difference was not statistically significant（ P〈0. 05）. Conclusion HH capsule has reliable anti- HBV effect,HPLC method is reliable for HH capsule quality control.

关 键 词：HH胶囊 指纹图谱 抗乙肝病毒 

分 类 号：R285.5[医药卫生—中药学]