补肾化痰法影响骨髓间充质干细胞成脂分化的实验研究  

The Experiment of the Effect of Adipogenic Differentiation of BMSCs on Bushen Huatan Therapy

作　　者：周亚娜[1] 陈辉[2] 向楠[2] 

机构地区：[1]湖北省中医院,湖北武汉430061 [2]湖北中医药大学,湖北武汉430065

出　　处：《时珍国医国药》2015年第10期2325-2327,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30672591);湖北省自然科学基金(No.2007ABA237)

摘　　要：目的研究补肾法、化痰法、补肾化痰法对BMSCs成脂分化的影响,探讨补肾化痰法治疗骨质疏松症的可能作用机制。方法运用全骨髓贴壁法分离培养纯化Wistar大鼠BMSCs,诱导BMSCs成脂分化,观察补肾中药、化痰中药和补肾化痰中药分别对BMSCs成脂分化后细胞形态学、油红O染色及定量和用RT-PCR检测脂蛋白脂酶(LPL)mRNA表达的影响。结果化痰中药能使BMSCs诱导形成的脂肪细胞减少,并下调成脂相关基因LPL-mRNA表达。结论化痰法可以抑制BMSCs向脂肪细胞分化。Objective To observe the effect of adipogenic differentiation of BMSCs on Bushen Therapy,Huatan Therapy,Bushen Huatan Therapy,and explore the mechanism of Bushen Huatan Therapy of treating osteoporosis. Methods BMSCs of Wistar rats were isolated,purified by using whole bone marrow adherent method. BMSCs was induced adipogenic differentiation. Through the observation of cytomorphology,oil red O staining and the expression of LPL- mRNA,it explored the effect of adipogenic differentiation of BMSCs on Bushen Therapy,Huatan Therapy,Bushen Huatan Therapy. Results Huatan Herb can make BMSCs reduce the generation of adipocyte,down- regulate the express- ion of LPL- mRNA. Conclusion Huatan Treatment can inhibit adipogenic differentiation of BMSCs.

关 键 词：补肾化痰法 骨质疏松症 骨髓间充质干细胞 成脂分化 

分 类 号：R285.5[医药卫生—中药学]