同一采集地不同采集时期首乌藤及叶中二苯乙烯苷含量的动态变化分析  

Determination of dynamic content of Stilbene glycoside in rattan and leaves of Polygonum multiflorum Thunb. picked in same growth environment during different growth period by HPLC

作　　者：朱培芳[1] 江舟[1] 赵荣华[2] 

机构地区：[1]云南中医学院实验中心,云南昆明650500 [2]云南中医学院中药学院,云南昆明650500

出　　处：《时珍国医国药》2015年第10期2501-2504,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81260553);南药研究协同创新中心资助项目

摘　　要：目的建立高效液相色谱法,对同一采集地不同采集时期首乌藤及叶中二苯乙烯苷含量的动态变化进行分析测定,为首乌藤资源的合理开发利用提供理论基础。方法色谱条件为：安捷伦1200系列高效液相色谱仪;Agilent SB-C18色谱柱（4.6mm×250mm,5μm）;流动相：采用乙腈-0.1%磷酸水溶液梯度洗脱;流速：1.0ml/min;检测波长：320nm;柱温：30℃,采用加热回流和超声提取两种方法对首乌藤及叶进行二苯乙烯苷提取。结果二苯乙烯苷进样量在3.33×10-3~66.67×10-3μg范围内呈现较好的线性关系,r=0.9999。平均回收率为98.907%,RSD为0.269%。结论同一生长环境不同采集时期首乌藤中二苯乙烯苷含量明显不同,变化显著,且不同采集时间首乌藤叶中没有测到二苯乙烯苷。此检测方法简单准确,高效,稳定性好,适用于首乌藤及叶中二苯乙烯苷含量的测定,实验结果为药典修订及首乌藤资源的合理开发利用提供理论基础。Objective To establish a method for determination of Stibene glucoside in the rattan and leaves of Polygonum multiflorum Thunb. by HPLC. Methods The Agilent 1200 series,Agilent SB- C18column（ 4. 6mm × 250 mm,5μm）,acetonitrile-o. 1% phosphoric acid,the flow rate was 1. 0m L / min,the wavelength of UV detector was set at 320 nm and column temperature was set at 30℃. Results The contents of Stibene glucoside present better regression equation and correlation coefficient,correlation coefficient reaches 0. 9999. Sample is in the range of 3. 33 ~ 66. 67 × 10- 3μg. The average recovery rate was 98. 907%. Conclusion The method is accurate,high precision and good stability. Experimental data provide a theoretical basis for Pharmacopoeia revision and rational exploitation of resources Polygoni multiflori Caulis.

关 键 词：首乌藤 二苯乙烯苷 高效液相色谱法 含量变化 

分 类 号：S567[农业科学—中草药栽培] R284.2[农业科学—作物学]