2007-2013年贵州省志贺菌分离株毒力相关基因的检测分析  

Detection and analysis on virulence-associated genes of Shigella isolated species in Guizhou Province, 2007-2013

作　　者：韦小瑜[1] 游旅[1] 田克诚[1] 马青[1] 刘英[1] 黄艳萍[1] 唐光鹏[1] 邹志霆[1] 王定明[1] 

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004

出　　处：《现代预防医学》2015年第21期3935-3938,共4页Modern Preventive Medicine

基　　金：国家科技重大专项(No.2012ZX10004-212)

摘　　要：目的了解2007-2013年间贵州省腹泻病例志贺菌分离株毒力相关基因携带情况及流行模式,为贵州省细菌性痢疾的预防和控制提供实验依据。方法采用PCR方法,对2007-2013年贵州省分离的178株志贺菌进行ipa H、ipa BCD、ial、sen和set1五种毒力相关基因检测,按血清型、年份和地区分析志贺菌中的毒力基因携带模式分布。结果178株志贺菌ipa H、ipa BCD、ial、sen和set1毒力基因的阳性率分别为100%、56.2%、59.6%、59.6%和18.0%,共有12种毒力基因携带模式,命名为Ⅰ-Ⅻ,Ⅻ型是福氏志贺菌的主要毒力基因携带模式(59.6%),Ⅷ型和Ⅰ型是宋内志贺菌的主要毒力基因携带模式(43.5%、42.0%);2007-2013年主要毒力基因携带模式依次为Ⅷ、Ⅻ、Ⅷ、Ⅰ、Ⅷ、Ⅰ和Ⅷ型;开阳、紫云以Ⅷ型为主要携带模式(55.2%、45.5%),平坝以Ⅻ型为主(80%),桐梓以Ⅰ型为主(51.0%)。结论 ipa H可作为志贺菌检测的特异基因,贵州省近年志贺菌毒力基因携带模式以Ⅷ、Ⅰ型为主,血清型不同、地区不同其主要毒力基因模式分布也不相同。Objective To study the prevalence modes of virulence-associated genes of Shigella species isolated from infectious diarrhea patients in Guizhou between 2007 and 2013, and provide scientific basis for bacillary dysentery control and prevention in Guizhou Province. Methods A total of 178 strains between 2007 and 2013 were detected the virulence-associated genes which included ipa H, ipa BCD, ial, sen and set1 by PCR. At the same time, the prevalence modes of virulence-associated genes were analyzed with serotypes, year and district. Results Among the 178 shigella strains, the positive rates of ipa H, ipa BCD, ial, sen and set1 were 100%, 56.2%, 59.6%, 59.6% and 18.0%, respectively. Based on their virulence gene distribution modes, the 178 shigella strains were divided into 12 groups, which were named from group Ⅰ to group Ⅻ. The group Ⅻ was the dominant mode for S.flexneri, accounting for 59.6%. While group Ⅷ andⅠwere the dominant modes for S.sonnei, accounting for 55.2% and 45.5%,respectively. Meanwhile, the groups of Ⅷ, Ⅻ, Ⅷ, Ⅰ, Ⅷ, Ⅰand Ⅷ were dominant modes between 2007 and 2013. The group Ⅷwas the dominant mode for Kaiyang and Ziyun County, accounting for 55.2% and 45.5%, respectively. While group Ⅻ was the dominant mode for Pingba County, accounting for 80% and the group Ⅰ was the dominant mode for Tongzi County, accounting for51.0%. Conclusion Ipa H could be used as a target gene for identification of Shigella spp. Group Ⅷ andⅠwere the dominant modes in recent years in Guizhou Province. The diverse distributions of the virulence-associated genes were according to the various distributions of strain types of Shigella and different districts.

关 键 词：志贺菌 PCR 毒力相关基因 

分 类 号：R117[医药卫生—公共卫生与预防医学]