胸腺素β4对增生性瘢痕基质金属蛋白酶-2及9表达作用的影响  

Effect of thymosin beta4 on matrix metalloproteinase-2,9 of hypertrophic scar

作　　者：樊志强[1] 廖立新[2] 张友来[1] 罗飞[2] 

机构地区：[1]南昌大学第一附属医院烧伤科,330000 [2]厦门大学第一附属医院烧伤整形科,361003

出　　处：《重庆医学》2015年第31期4321-4323,4326,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30660234)

摘　　要：目的 研究胸腺素β4对人增生性瘢痕成纤维细胞中基质金属蛋白酶-2（MMP-2）和MMP-9的表达影响,探讨胸腺素β4对增生性瘢痕的作用机制。方法 2013年6-9月在南昌大学第一附属医院烧伤整形科手术切取的增生性瘢痕组织（烧伤后6个月内）,体外培养成纤维细胞,分为实验组（胸腺素浓度分别为0.05、0.1、1.0、5.0μg/mL）,对照组（不添加胸腺素β4）,采用反转录-聚合酶链反应（RT-PCR）以及蛋白免疫印迹法（Western blot）测定实验组和对照组的MMP-2、MMP-9表达变化。结果胸腺素β4作用后增生性瘢痕组织内MMP-2、MMP-9水平增加,分别以1.0μg/mL实验组和5.0μg/mL实验组作用最明显,与对照组比较差异均有统计学意义（P〈0.05）。结论 胸腺素β4能剂量依赖性促进MMP-2、MMP-9表达,可能是抑制增生性瘢痕的作用因素之一。Objective To study the effect of thymosin beta4 on the expression of matrix metalloproteinase-2（MMP-2） and MMP-9 of human hypertrophic and explore mechanism of thymosin beta4 on hypertrophic scar. Methods Fibroblasts were cultured in vitro ic patients（within 6 months after burns） admitted in the first affiliated hospital of Nanchang university from Ju： 2013. The Fibroblasts were divided into experiment group：0.05,0. 1,1, 0μg/mL and 5.0 μg/mL,and control grou eta4. The expression levels of MMP-2-,MMP-9 were observed by RT-PCR and Western blot. Results The expression MMP-2 and MMP-9 were increased after the thymosin beta4 took effect , and it was especially significant in 1. 0 μg/mL and 5. 0μg/mL group,there was statistical difference between experlment group and control group （P〈0. 05）. Concision Thymosin beta4 can dose-dependently promote the expression of MMP-2 and MMP-9 and inhibit the pro-liferation of hypertrophic scar.

关 键 词：胸腺素 瘢痕 基质金属蛋白酶-2 基质金属蛋白酶-9 细胞外基质 

分 类 号：R622[医药卫生—整形外科]