甘氨酸补充对力竭性运动小鼠部分生化指标的影响  

Influence of glycine supplement on partial biochemical indicator in exhausive exercise mice

作　　者：曹进[1] 樊晓飞[2] 李良菊[1] 徐姿 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏大学体育部,江苏镇江212013 [3]江苏省昆山市第一人民医院检验科,215300

出　　处：《重庆医学》2015年第31期4327-4329,4332,共4页Chongqing medicine

基　　金：中国博士后科学基金项目资助(2015M571706)

摘　　要：目的探讨甘氨酸补充对一次力竭性运动小鼠部分生化指标的影响。方法将雄性昆明小鼠分为3组,每组10只:力竭性游泳运动组(E组),甘氨酸补充后力竭性游泳组(G组)及静息正常对照组(S组)。G组小鼠采用甘氨酸灌胃处理4周,其余两组给予生理盐水。4周后,E组和G组均做力竭性游泳运动。采样后进行补体溶血活性测定,并测定血清中部分生化指标的水平。结果与E组比较,G组的力竭性游泳运动时间显著延长(P<0.01)。力竭性游泳后,E组小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、尿素氮、血清铁(Fe3+)和补体溶血活性(CHA)的水平较S组发生了显著性的变化(P<0.01),与E组比较,G组小鼠血清中上述生化指标得到了显著改善(P<0.01),其中ALP和LDH的水平可以恢复到与S组相当的水平(P>0.05)。结论力竭性游泳小鼠血清部分生化指标异常,甘氨酸预补充对这些异常有明显的改善作用,可以一定程度地提高小鼠的运动能力。Objective To investigate the influence of glycine supplement on partial biochemical indicator in exhaustive exercise mice. Methods Divided Kunming＇s male-mice into 3 group（10/group） .. exhaustive swimming group（E） ,exhaustive swimming group with glycine supplement（G） and silent control group （S）. Mice in the G group was treated with 4-weeked intragastric administration of glycine, while mice in other 2 groups received physiological saline. After 4 weeks.mice in E and G group did the exhaustive swimming exercise. After sampling, the activity of complement hemolytic activity was measured, and the serum levels of some biochemical indexes were determined. Results Time of exhaustive swimming in G group was significantly longer than E group（P〈 0.01）. After exhaustive swimming, the ALT, AST,ALP, LDH,CK, N, Fe^3＋ and CHA in E group were significantly changed compared with S group（P〈0.01）; while those indicators in G group were significantly improved than E group（P〈0.01）, the ALP and LDH level can regain to the level of S group（P〈0. 05）. Conclusion Exhaustive swimming mice can have part indicator abnormality,but Glycine supplement can effectively improve that condition and enhance the exercise ability to some extent.

关 键 词：甘氨酸 力竭性游泳 生化指标 小鼠 近交系 

分 类 号：R87[医药卫生—运动医学]